Antimicrobial Susceptibility of Bovine Subclinical Mastitis and During Milk Separation Isolates and the Accompanying Hygienic Practices

Sultan Farag Nagati,Safaa Khamis Hassan

doi:10.3844/ajavsp.2021.112.123

Abstract

Milk is considered one of the most perfect foods for humans and milk contains many nutrients. Subclinical mastitis has been counted as a great problem for dairy manufacture. The disease causes severe economic losses due to lower milk production, treatment costs and increased labor and milk retained for human consumption after treatment and premature culling. A cross-sectional study was conducted in 3 villages of Tamia district, Fayoum governorate, Bacteriological examination, Antibiotic susceptibility test, to study the presence of sub-clinical mastitis and milk contamination during milk separation and milking process and to detect the most resistance isolate to antibacterial agents and find the suitable antibacterial and disinfectants. 25.7% of the milk samples were positive for the California test. Skim milk and cream showed the isolation of Staphylococcus aureus (S. aureus), Streptococcus aglacteae (S. aglacteae), Coliform bacteria, Arcanobacterium pyogenes (A. pyogenes) and Escherichia coli (E. coli). The microbiological quality of the two disinfectants tested was considered satisfactory, as no significant number of microbial contaminants were recovered from them following the sterility test. Only 26.7% of milkers use water and soap for washing their hands. The participants had a lack of information about subclinical mastitis and improper hygienic practices during the milking and milk-handling process.

Highlights

Milk is considered one of the most perfect foods for humans, milk contains many nutrients needed for growth and development and prevention of many nutritional disorders (Dhanashekar et al, 2012)
Machines one and six used milk with subclinical mastitis of both types of animals, Machines two and four used milk of the same animal with no subclinical mastitis, Machine three used milk of the same animal with subclinical mastitis and Machine 5 used milk both animals with no subclinical mastitis. 74.3% of milk samples used in this study were negative to the California test, which clarified that most of the milk samples were non-mastitic milk, while 25.7% were positive for the California test Table 3
The isolation of different pathogens in cow and buffalos milk corroborate the results of the California test, which discovered the presence of a subclinical mastitis Tables 4 and 6

Summary

Introduction

Milk is considered one of the most perfect foods for humans, milk contains many nutrients needed for growth and development and prevention of many nutritional disorders (Dhanashekar et al, 2012). Raw milk is still used by a large number of farm families, workers and growing sectors of the population because they believe this raw milk is safe, but pasteurization destroys the health benefits of milk (LeJeune and Rajala-Schultz., 2009) milk is rich in proteins, lipids and sugars, milk is an example of ideal culture medium for various pathogens, including bacteria, viruses, fungi and parasites, which can be important sources of foodborne pathogens These pathogens could be found in milk by direct contact with the contaminated animal environment and secretion from the udder of infected animals including mastitis and subclinical mastitis (Dhanashekar et al, 2012; Zeinhom and Abdel-Latef, 2014). Over 100 diverse micro-organisms have been listed as a cause of intramammary infection in dairy animals, the main causative micro-organisms were classified into infectious pathogens including (Staphylococcus aureus and Streptococcus agalactiae) and environmental organisms most encountered are species of streptococci other than Streptococcus

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