Antimicrobial susceptibility and virulence-associated genes in Campylobacter isolates from milk and wastewater in Hatay, Turkey

Mehmet Elmalı,Hayriye Yeşim Can

doi:10.1590/0103-8478cr20180227

Mehmet Elmalı, Hayriye Yeşim Can

Open Access

https://doi.org/10.1590/0103-8478cr20180227

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Journal: Ciência Rural	Publication Date: Jan 1, 2019
Citations: 12	License type: CC BY 4.0

Affiliation: Mustafa Kemal University

Abstract

ABSTRACT: Campylobacter is one of the most common causes of bacterial foodborne diseases throughout the world. This study was conducted to determine the prevalence, antimicrobial resistance and virulence of Campylobacter isolates of raw cow’s milk and cattle slaughterhouse wastewater samples in Hatay, Turkey. A total of 114 raw milk and 78 wastewater samples were analyzed for the identification of C. jejuni, C. coli, and C. lari by multiplex PCR. The overall prevalence of Campylobacter was found to be 7.2%, of these isolates, 85.7% were identified as C. jejuni and 14.2% as C. coli, but C. lari was not detected in the study. The cdtA and cadF genes were present in 66.6% and 41.6% of C. jejuni isolates tested, respectively, but wlaN gene was not found in any of the isolates. Results of antimicrobial resistance analysis showed that 71.4% of the isolates were resistant to erythromycin, 64.2% to tetracycline, and 57.1% to ciprofloxacin. Overall, 8 of 14 Campylobacter isolates (57.1%) showed a multidrug resistance.

Highlights

Campylobacters are zoonotic, and main reservoirs of them are wild and domestic animals, especially intestinal tract of birds
Its products are considered as the common source of Campylobacter infection in humans (PEZZOTTI et al, 2003; PÉREZ-BOTO et al, 2010; DUARTE et al, 2014; BOLTON, 2015; WIECZOREK & OSEK, 2015; ZHONG et al, 2016), and cattle may play an important role for human campylobacteriosis
Antimicrobial resistance and virulence genes of Campylobacter isolates recovered from raw milk and slaughterhouse wastewater samples were investigated in this study

Summary

INTRODUCTION

Campylobacters are zoonotic, and main reservoirs of them are wild and domestic animals, especially intestinal tract of birds. Its products are considered as the common source of Campylobacter infection in humans (PEZZOTTI et al, 2003; PÉREZ-BOTO et al, 2010; DUARTE et al, 2014; BOLTON, 2015; WIECZOREK & OSEK, 2015; ZHONG et al, 2016), and cattle may play an important role for human campylobacteriosis. Since the poultry and poultry meat are the most important sources of Campylobacter infections, many authors have studied the prevalence of Campylobacter on poultry in different countries (ZANETTI et al, 1996; DOMÍNGUEZ et al, 2002; PEZZOTTI et al, 2003; YANG et al, 2003; WHYTE et al, 2004; HUSSAIN et al, 2007; BARDOŇ et al, 2011; ZENDEHBAD et al, 2015; ZHONG et al, 2016). Antimicrobial resistance and virulence genes of Campylobacter isolates recovered from raw milk and slaughterhouse wastewater samples were investigated in this study

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION