Antimicrobial sensitivity of Avibacterium paragallinarum isolates from layers in the special region of Yogyakarta, Indonesia.

Ima Fauziah,Widya Asmara,Agnesia Endang Tri Hastuti Wahyuni

doi:10.14202/vetworld.2021.1124-1127

Ima Fauziah, Widya Asmara + Show 1 more

Open Access

https://doi.org/10.14202/vetworld.2021.1124-1127

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Journal: Veterinary world	Publication Date: May 1, 2021
Citations: 4	License type: CC BY 4.0

Affiliation: Universitas Gadjah Mada

Abstract

Background and Aim:Infectious coryza (IC) is an upper respiratory disease of chicken caused by Avibacterium paragallinarum. Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to determine the antimicrobial sensitivity of A. paragallinarum isolates from layers in the Special Region of Yogyakarta, Indonesia.Materials and Methods:The samples used in this study were 30 layers that showed IC symptoms. The colony and cell morphology were observed with Gram staining; then, biochemical tests (catalase, oxidase, urease, indole, and motility tests, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) were performed to the suspected colony to identify A. paragallinarum. An antibiotic sensitivity test was performed using several antibiotic disks against A. paragallinarum isolates that were cultured on Mueller-Hinton Agar.Results:Out of 30 samples, 24 samples (80%) were found positive for A. paragallinarum. All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%). The antibiotics with intermediate sensitivity were enrofloxacin (79.2%), fosfomycin (75%), and ciprofloxacin (54.2%). The isolates were most resistant to erythromycin (100%), followed by tetracycline (87.5%), streptomycin (83.3%), doxycycline and kanamycin (70.8%), and trimethoprim (62.5%).Conclusion:Out of the total samples, 24 samples (80%) from layers with IC symptoms were identified biochemically as A. paragallinarum. It was sensitive to AMP, AML, and C.

Highlights

Avibacterium paragallinarum is a Gram-negative bacterium that causes infectious coryza (IC), an upper respiratory tract disease in chickens [1,2]
All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%)
The samples were collected from 30 layers with IC symptoms and with no antibiotic treatment after infection

Summary

Introduction

Avibacterium paragallinarum is a Gram-negative bacterium that causes infectious coryza (IC), an upper respiratory tract disease in chickens [1,2]. The most common symptoms of IC are facial swelling, infraorbital edema, conjunctivitis, and malodorous nasal and ocular discharge [3]. IC occurs worldwide and causes great economic losses due to decreased egg production (up to 40%), increased culling rate of chickens, and increased medical costs [4]. An appropriate selection and use of antibiotics for IC treatment must be considered because they can only reduce the clinical signs of IC but not cure the disease completely [6]. Infectious coryza (IC) is an upper respiratory disease of chicken caused by Avibacterium paragallinarum. Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to determine the antimicrobial sensitivity of A. paragallinarum isolates from layers in the Special Region of Yogyakarta, Indonesia

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