Antimicrobial Screening of Endophytic Fungi Isolated from the Aerial Parts of Paepalanthus chiquitensis (Eriocaulaceae) Led to the Isolation of Secondary Metabolites Produced by Fusarium fujikuroi

Felipe Hilário,Paulo Sano,Angela Araujo,Vanessa Chapla,Taís Bauab,Lourdes Dos Santos

doi:10.21577/0103-5053.20160312

Felipe Hilário, Paulo Sano + Show 4 more

Open Access

https://doi.org/10.21577/0103-5053.20160312

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Abstract

The screening for antimicrobial activity of twenty five endophytic fungi isolated from the aerial parts of Paepalanthus chiquitensis (Eriocaulaceae) was assayed against the bacteria Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli and Salmonella setubal, and the yeast fluconazole-resistant Candida albicans. The ethyl acetate extract produced by Fusarium fujikuroi was the most bioactive and this fungus was chosen for the chemical study, affording the isolation of an alkaloid 2-(4-butylpicolinamide) acetic acid and three known metabolites: fusaric acid, indole acetic acid and the sesterterpene terpestacin. The minimal inhibitory concentration of the extract and of fusaric acid and indole acetic acid for all the tested microorganisms had values from 125 to 1000 µg mL-1.

Highlights

The Paepalanthus chiquitensis Herzog (synonym Paepalanthus giganteus Sano)[1] represents one of the 1200 species belonging to the Eriocaulaceae
The Paepalanthus chiquitensis Herzog[1] represents one of the 1200 species belonging to the Eriocaulaceae
The screening for antimicrobial activity was used as a bioassay-guided strategy to select the most active among the twenty-five EtOAc extracts prepared from endophytic fungi isolated from the aerial parts of P. chiquitensis

Summary

Introduction

The Paepalanthus chiquitensis Herzog (synonym Paepalanthus giganteus Sano)[1] represents one of the 1200 species belonging to the Eriocaulaceae. The investigation of the EtOAc extract resulted in the isolation and structure elucidation of four metabolites produced by F. fujikuroi: fusaric acid (1), indole acetic acid (2), 2-(4-butylpicolinamide) acetic acid (3) and the sesterterpene terpestacin (4). The antibacterial and antifungal activities of the EtOAc extract and major isolated compounds (1) and (2) have been described.

Results

Conclusion