Abstract
Background and Aim:Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are gradually increasing worldwide and carry a serious public threat. This study aimed to determine the antimicrobial resistance pattern of ESBL-producing E. coli isolated from fecal samples of piglets and pig farm workers.Materials and Methods:Fecal samples from <3-month-old piglets (n=156) and farm workers (n=21) were processed for the isolation of ESBL-producing E. coli in MacConkey agar added with 1 µg/mL of cefotaxime. E. coli (piglets=124; farm workers=21) were tested for ESBL production by combined disk method and ESBL E-strip test. Each of the ESBL-positive isolate was subjected to antibiotic susceptibility testing. The ESBL-producing E. coli were further processed for genotypic confirmation to CTX-M gene.Results:A total of 55 (44.4%, 55/124) and nine (42.9%, 9/21) ESBL-producing E. coli were isolated from piglets and farm workers, respectively. Antibiotic susceptibility testing of the ESBL-positive E. coli isolates from piglets and farm workers showed 100% resistance to ceftazidime, cefotaxime, cefotaxime/clavulanic acid, ceftazidime/clavulanic acid, and cefpodoxime. A proportion of 100% (55/55) and 88.9% (8/9) ESBL-positive E. coli were multidrug resistance (MDR) in piglets and farm workers, respectively. On genotypic screening of the ESBL E. coli isolated from piglets (n=55), 15 were positive for the blaCTX-M gene and of the nine ESBL E. coli from farm workers, none were positive for the blaCTX-M gene.Conclusion:Although there was no significant difference in isolation of ESBL-producing E. coli between piglets and farm workers, the ESBL-positive E. coli from piglets showed relatively higher MDR than farm workers.
Highlights
Treating infections associated with extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are becoming difficult since they are capable of hydrolyzing penicillin, broad-spectrum cephalosporins, and monobactams and are often resistant to other antimicrobial classes such as fluoroquinolones, aminoglycosides, and trimethoprim-sulfamethoxazole [1]
On genotypic screening of the ESBL E. coli isolated from piglets (n=55), 15 were positive for the blaCTX-M gene and of the nine ESBL E. coli from farm workers, none were positive for the blaCTX-M gene
Conclusion: there was no significant difference in isolation of ESBL-producing E. coli between piglets and farm workers, the ESBL-positive E. coli from piglets showed relatively higher multidrug resistance (MDR) than farm workers
Summary
Treating infections associated with extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are becoming difficult since they are capable of hydrolyzing penicillin, broad-spectrum cephalosporins, and monobactams and are often resistant to other antimicrobial classes such as fluoroquinolones, aminoglycosides, and trimethoprim-sulfamethoxazole [1]. Reports suggest the possibility of transferring ESBL-producing E. coli from farm workers to food animal and vice versa [6,7,8,9,10]. Multidrug-resistant E. coli are reported in food animals and pet animals of India [11,12,13,14,15]. Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are gradually increasing worldwide and carry a serious public threat. This study aimed to determine the antimicrobial resistance pattern of ESBL-producing E. coli isolated from fecal samples of piglets and pig farm workers
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