ANTIMICROBIAL RESISTANCE IN ENTEROBACTERIACEAE FROM INTENSIVELY-REARED APPARENTLY HEALTHY AND DISEASED POULTRY IN ABEOKUTA, NIGERIA

Abstract

The emergence and wide-spread dissemination of antimicrobial resistant bacteria strains is a global phenomenon of great public health and economic implications. Antimicrobial resistance was investigated in enterobacteriaceae isolated from apparently healthy and diseased poultry birds using the broth micro-dilution method to determine antimicrobial minimum inhibitory concentration (MIC). In all, 504 bacterial isolates including Escherichia coli (471), Klebsiella spp (28) and Salmonella enterica isolates (5) were studied. The isolates were resistant to ampicillin (88.5%), chloramphenicol (62.3%), ciprofloxacin (74.8%), enrofloxacin (81.0%), neomycin (83.9%), norfloxacin (78.8%), streptomycin (91.3%) and tetracycline (83.3%). The geometric mean MIC (µg/µL) of tested antimicrobials for enterobacteriaceae is as follows: ampicillin (102.5), chloramphenicol (48.4), ciprofloxacin (19.1), enrofloxacin (34.5), neomycin (47.7), norfloxacin (24.5), streptomycin (142.2) and tetracycline (62.5). Although rates of resistance to ampillin, streptomycin and tetracycline were similar among isolates from apparently healthy and diseases birds, resistance to chloramphenicol, ciprofloxacin, enrofloxacin, neomycin and norfloxacin were significantly higher (p<0.05) in isolates from diseased chickens than in those from apparently healthy chickens. The high rates of antimicrobial resistance in bacteria may contribute to the persistence of pathogens in poultry flock and ineffectiveness of antimicrobial chemotherapy during disease outbreaks.Â