Antimicrobial Property of Lemongrass Oil against Porphyromonas Gingivalis

Abstract

Aim: This study determines molecular docking to know the selected active compounds of lemon grass against bacterial receptor proteins of Porphyromonasgingivalis and the minimum inhibitory concentration of lemon grass extract against P. gingivalis. Materials and methods: The lemon grass essential oil extraction was subjected for evaporation to remove the methanol and was collected for analysis by Gas Chromatography Mass Spectrometry Analysis (GCMS) to know the compounds of lemon grass which was effective against peptidyl-arginine deiminase of P. gingivalis. Molecular docking technique was done to study these compounds and their interactions with the target protein in the test organism. Finally, minimum inhibitory concentration was done to know the antibacterial activity of lemon grass oil components against P. gingivalis. Results: A total of 15 compounds were obtained after GCMS analysis but 3 main compounds were selected i.eCitronellol, D-Limonene and Geraniol. D- limomonene exhibited good binding with a score of -6.05. Geraniol and Citronellol exhibited a binding score of -5.84 and -5.32 respectively for P.gingivalis. 200µl of extract of lemon grass caused 50.2% reduction in number of colonies of P. gingivalis Conclusion: The active ingredients from lemongrass oil containing Citronellol, D-Limonene and Geraniol showed antibacterial activity against P. gingivalis with MIC of 20µl/ml.