Antimicrobial Properties, Phytochemical Composition, and Phenotypic Resistance Pattern of Selected Enteropathogenic Microorganism on Ageratum conyzoides (Goat Weed) Leaf Extract

Abstract

The aim of this study is to determine the phytochemical composition, antimicrobial properties and phenotypic resistance pattern of selected enteropathogenic microorganism on Ageratum conyzoides leaf extract. Enteropathogenic isolates were collected from Adekunle Ajasin University Health Centre, Akungba Akoko, Ondo State and were identified using Microbact™ 24E Identification kit. Antibiotic sensitivity testing was carried out against the enteropathogenic organism using Kirby-Bauer disc diffusion method, imipenem was active against Proteus mirabilis, Enterobacter agglomerans, Enterobacter gerogoviae, with diameter zone of inhibition of 41 mm, 26 mm and 30 mm. ofloxacin were active against Proteus mirabilis, Enterobacter agglomerans, Enterobacter gergoviae, with diameter zone of inhibition of 21, 31 and 21 mm. All enteropathogenic organisms shows high resistant rate against oxacilin, vancomycin, amoxicillin, ceftazidime, piperacillin, cefoxitin, tazobactam. Antimicrobial screening of the leaf extract of Ageratum conyzoides revealed high bioactivity against Serratia marcescens, Proteus vulgaris, Enterobacter agglomerans and Proteus mirabilis isolates with diameter zone of inhibition ranging from 15 mm to 22 mm at 100 mg/ml. Using broth dilution method, the minimum inhibitory concentrations (MIC) of the Ageratum conyzoides (Goatweed) extract range from 25 mg/ml and 100 mg/ml. The phytochemical screening results shows that Ageratum conyzoides extract contained alkaloid, pholobotannins, Cardiac glycoside, phenol, tannins, saponin, and flavonoids. It can be deduced from this research work that Ageratum conyzoides has better antimicrobial properties and it contains very important phytochemical constituents which can be a magic bullet to deal with the menace of antimicrobial resistance enteropathogenic microorganism.