Antimicrobial properties of tissue conditioner containing silver doped bioactive glass nanoparticles: in vitro study

Abstract

The objective of this work is to prepare and verify the antimicrobial properties of tissue conditioner containing silver doped bioactive glass nanoparticles (Ag-BG NPs) in vitro against three oral pathogens. A novel bioactive glass (BG) nanoparticles (NPs) with different Ag weight percentages of 0.6, 1.2, 3 and 4 wt% was prepared via a modified sol-gel method. Characterization of Ag-BG NPs was carried out using transmission electron microscopy (TEM), Fourier transformer infrared spectrophotometer (FTIR). Antimicrobial activities of the NPs were estimated. Also, ions release was measured using an inductively coupled plasma optical emission spectroscopy (ICP/OES). The 4% Ag-BG NPs was incorporated into the tissue conditioner (TC) for the preparation of the composite specimens (TC/Ag BG 4%). The dispersion of the NPs was evaluated using SEM/EDX. ion release and antimicrobial activities were evaluated. Finally, cell cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on normal human fibroblast (BJ1). Data analysis was performed using one-way ANOVA, and Tukey’s post hoc test. The Ag-BG NPs (4%) exhibited the highest statistical significant mean inhibition value against selected pathogens. A novel Ag-BG NPs with antimicrobial properties with gradual release of were developed. However, the antimicrobial activity was lost when it was incorporated into the tissue conditioner.