Abstract
ABSTRACTPolymeric films were coated with nisin, an antimicrobial peptide, for potential application in shelf‐life extension of food. Five commercial packaging films with varying surface energies were compared. The highest antimicrobial activity was observed on the most hydrophobic nisin‐coated films (surface energy ± 32 dyne/cm). The binding affinity of nisin to the surface of ethylene acrylic acid copolymer (surface energy = 32 dyne/cm) was investigated. Films were coated in nisin solutions under varying conditions including nisin concentration, contact time, solution's volume to film area ratio, pH of coating solution and temperature during coating. Stronger nisin activity was detected on films as the coating time increased from 30 s to 2 h. Higher nisin coating concentration also promoted the binding of nisin to films with saturation level observed at 250 μg/mL. Films coated with nisin using a higher solution to film area ratio (0.84 compared to 0.14 and 0.28 mL/cm2) also exhibited higher nisin activity.
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