Antimicrobial potential of different medicinal plants against food industry pathogens

Miroslava Kačániová,Eva Ivanišová,Dzmitry Hrinshpan,Lucia Galovičová,Jana Žiarovská,Petra Borotová,Katarí­Na Rovná,Veronika Valková,Simona Kunová,Tatsiana Savitskaya

doi:10.5219/1387

Abstract

Work aimed to investigate the antimicrobial activity of medicinal plants against selected species of food industry pathogens in vitro conditions. The detection of antibacterial properties was examined by the disc diffusion method and the method of the minimum inhibitory concentrations (MIC). The cultivation of microorganisms after the 24 h was performed by disc diffusion method. Petri dishes have grown at 37 °C in which the Mueller - Hinton agar and application it to the sterile paper disc impregnated with the extract. The thickness of the resulting inhibition zone was measured with a ruler after completion of the culture. After the preparation of bacteria and extracts of certain concentrations of a subsequently added to wells microplates we use the method of the minimum inhibitory concentration (MIC) which was conducted out as the second measurement, and we took the readings absorbance spectrophotometer at 570 nm using the Glomax plate spectrophotometer. We found out, that Equisetum arvense demonstrated the largest zones of inhibition to the tested Gram-positive and Gram-negative bacteria. The greatest antimicrobial activity achieved Equisetum arvense, Urtica dioica, and Taraxacum officinale against Salmonella enterica subsp. enterica CCM 3807 and Yersinia enterocolitica CCM 5671. Equisetum arvense and Taraxacum officinale was the most effective against Escherichia coli CCM 2024 and the least effective were Tussilago farfara and Mentha piperita with using the method of minimum inhibitory concentrations.

Highlights

Salvia officinalis belongs to the largest genus of the family Lamiaceae containing approximately 900 different species worldwide
That Equisetum arvense demonstrated the largest zones of inhibition to the tested Grampositive and Gram-negative bacteria
Disk diffusion method (Table 1) indicated that E. coli, S. enterica, and Y. enterocolitica were more sensitive to extracts from selected medicinal plants than L. monocytogenes and S. aureus

Summary

Introduction

Salvia officinalis belongs to the largest genus of the family Lamiaceae containing approximately 900 different species worldwide. Several studies have described different biological effects of Equisetum arvense L., Tussilago farfara, Melissa officinalis, Urtica dioica, Salvia officinalis and Mentha piperita extract or tea with natural extract, such as antioxidant, anti-inflammatory, antibacterial, antifungal, vasorelaxant, neuro and cardio protectors (Dos Santos et al, 2005; Sandhu et al, 2010; Salehzadeh et al, 2014; Salih, 2014; Rabbani et al, 2015; Lee et al, 2019), and antiproliferative properties (Yamamoto, Inoue and Hamako, 2004; Četojevic-Simin et al, 2010; Stanojevic et al, 2010). Disk diffusion method (Table 1) indicated that E. coli, S. enterica, and Y. enterocolitica were more sensitive to extracts from selected medicinal plants than L. monocytogenes and S. aureus.

Results

Conclusion