Antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles.

Ashraf M M Essa,Mohamed K Khallaf

doi:10.1186/s12866-016-0766-8

Ashraf M M Essa, Mohamed K Khallaf

Open Access

https://doi.org/10.1186/s12866-016-0766-8

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Journal: BMC microbiology	Publication Date: Jul 11, 2016
Citations: 29	License type: cc-by

Affiliation: Fayoum University, Jazan University

Abstract

BackgroundBiodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.MethodsCopper nanoparticles (CuNPs) were prepared biologically using the headspace gases generated by the bacterial culture Escherichia coli Z1. The antimicrobial activity of CuNPs was evaluated against the bacterial strains Bacillus subtilis, Micrococcus luteus, Streptomyces parvulus, Escherichia coli, Pseudomonas aeruginosa as well as some fungal strains Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Fusarium solani and Alternaria solani.ResultsBiological CuNPs demonstrated antibacterial and antifungal activities higher than those of the untreated copper sulfate. At the same time, limestone and sandstone blocks treated with consolidation polymers functionalized with CuNPs recorded apparent antimicrobial activity against E. coli, S. parvulus and B. subtilis in addition to an improvement in the physical and mechanical characters of the treated stones. Furthermore, the elemental composition of CuNPs was elucidated using electron dispersive x-ray system connected with the scanning electron microscope.ConclusionConsolidation polymers impregnated with CuNPs could be used to restrain microbial deterioration in addition to the refinement of physico-mechanical behavior of the historic stones.

Highlights

Biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix
The antibacterial potentiality of the collected Cu-particles was demonstrated at different concentrations
There was no bacterial growth at the concentration 150 μg/mL or above while at 100 μg/mL the percentage of growth reduction reached 94.7 % for E. coli, 92.4 % for M. luteus, 90.9 % for S. parvulus, and 95.5 % for B. subtilis

Summary

Introduction

Biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix. It has been reported that nanoparticles of Ag, Zn, Cu and Au exhibit a wide spectrum of antimicrobial activity against different bacterial [3,4,5] and fungal species [6,7,8,9]. Copper nanoparticles were reported to have antimicrobial activity against wide spectrum of bacteria. Elevated levels of heavy metals represent a potential stimulus for metal tolerant bacteria that regularly possess specific metal resistance mechanisms. One of these mechanisms is the intracellular or extracellular transformation of metal ions into insoluble metal particles [15, 16]. Bacteria can release certain metabolites into their microenvironment that can transform metal ions into less

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