Antimicrobial Metabolites from the Endophytic Fungus Aspergillus sp. of Eucommia ulmoides

Abstract

Endophytic fungi are eukaryotic organisms that live inside plant tissues and are usually specific at the host species level [1]. They have proven to be a rich source of novel organic compounds with interesting biological activities and a high level of biodiversity [2]. In the course of our search for biologically active metabolites from endophytic fungi from Chinese medicinal plants, a subculture of an isolate of Aspergillus sp., obtained from roots of Eucommia ulmoides, was cultivated on potato dextrose agar (PDA). An ethyl acetate extract of the culture showed significant antimicrobial activity. This prompted us to carry out secondary metabolites studies on this fungus, which resulted in the isolation of seven compounds. Herein we describe the isolation, structural elucidation, and antimicrobial activities of its secondary metabolites. The fungal strain ER15 was isolated from the roots of Eucommia ulmoides, collected in the Qinling Mountains, Shaanxi Province, China, on September 10, 2010. The fungus was identified as a member of the genus Aspergillus by DNA amplification and sequencing of the ITS region. The fungal strain has been preserved at the Shanxi Datong University, Shanxi Province, China. Starter cultures were maintained on PDA medium at 28 C for 7 days. Plugs of agar supporting mycelial growth were cut and transferred aseptically to 1000 mL Erlenmeyer flasks containing 400 mL of liquid Czapek medium at 28 C on a rotary shaker set to 120 r/min for 15 days. The fungal culture (60 L) was filtered through cheesecloth. The filtrate was concentrated to 10 L below 60 C and then extracted five times with ethyl acetate (15 L). The dried mycelium (55 C, 95 g) was extracted three times with methanol (4 L). All extracts were concentrated at reduced pressure to afford 12.1 g of a crude extract. The crude extract was separated into eight fractions (A–H) on silica gel (300 g) using gradients of ethyl acetate–methanol (1:0, 100:1, 50:1, 20:1, 10:1, 5:1, 2:1, 0:1). Fraction A was separated by silica gel column chromatography eluting with petroleum ether– ethyl acetate (1:2) to give pure compound 1 (87 mg), together with crude compound 2. The crude compound 2 was then recrystallized from ethyl acetate–methanol to give the pure compound 2 (11 mg). Fraction B was separated by column chromatography on silica gel with a gradient of ethyl acetate in petroleum ether to give five subfractions (B1-5). Fraction B3 was further separated by silica gel column chromatography and eluted with a gradient of ethyl acetate in petroleum ether and Sephadex LH-20 with ethyl acetate–methanol (1:1) to give compounds 3 (118 mg), 4 (27 mg), and 5 (19 mg). Similarly, compounds 6 (10 mg) and 7 (37 mg) were isolated from fraction B4 after elution with a gradient of ethyl acetate in petroleum ether and Sephadex LH-20 with ethyl acetate–methanol (1:1). The secondary metabolites were identified as ergosterol (1) [3], cerevisterol (2) [4], 5-hydroxymethylfuran-3-carboxylic acid (3) [5], 5-methoxymethylfuran-3-carboxylic acid (4) [6], allantoin (5) [7], trypacidin (6) [8], and monomethylsulochrin (7) [9] by comparison of their spectral data with the reported data in the literature.