Abstract
Antimicrobial bioassay-guided fractionation of the endophytic fungi Neofusicoccum australe led to the isolation of a new unsymmetrical naphthoquinone dimer, neofusnaphthoquinone B (1), along with four known natural products (2–5). Structure elucidation was conducted by nuclear magnetic resonance (NMR) spectroscopic methods, and the antimicrobial activity of all the natural products was investigated, revealing 1 to be moderately active towards methicillin-resistant Staphylococcus aureus (MRSA) with a minimum inhibitory concentration (MIC) of 16 µg/mL.
Highlights
In 2014, the World Health Organization (WHO) described how drug-resistant microbes are present in every region of the world [1]
Pre-screening of the International Collection of Microorganisms from Plants (ICMP) fungal cultures for antimicrobial activity involves briefly growing the cultures on potato dextrose agar (PDA) before small wells are cut into the agar and each well inoculated with 5 × 106 colony forming units of luciferase-tagged derivatives of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus
The cultures are incubated, and the inhibitory activity of the ICMP isolates monitored by the extent of reduction in bacterial light production compared to bacteria isolated with no fungus
Summary
In 2014, the World Health Organization (WHO) described how drug-resistant microbes are present in every region of the world [1]. The report concluded that within a decade, antimicrobial resistance will make routine surgery, organ transplantation, and cancer treatment life-threateningly risky [1]. Key to managing this crisis is to boost the number of new antibiotic classes reaching the clinic [2,3]. The isolation, structure elucidation and bioactivity of neofusnaphthoquinone B (1) are described. Included two naphthalene monomers (2 and 3) [10], pramanicin A (4) [11] and 4-hydroxyscytalone (5) [8], was conducted by comparison of 11H NMR data with those reported in the literature. The isolation, structure elucidation and bioactivity of neofusnaph of 10 thoquinone B (1) are described
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