Antimicrobial efficacy of grape seed extract against Escherichia coli O157:H7 growth, motility and Shiga toxin production

Abstract

Escherichia coli O157:H7 produces Shiga toxin (Stx) which is heat stable and causes Hemolytic Uremic Syndrome (HUS), a serious disease associated with bloody diarrhea and even death. To ensure food safety, both live E. coli O157:H7 and its toxin production in food products need to be controlled. Natural ingredients with inhibitory effects on E. coli O157:H7 growth and toxin production are top choices of antimicrobials for the food industry. The objectives of this study were to evaluate efficacy of grape seed extract (GSE) against the growth, swimming motility and Stx production of E. coli O157:H7. The disc diffusion assay indicated that 3.2 mg GSE per disc resulted in an inhibition zone of 14.8 ± 0.21 mm. The minimal inhibitory concentration of GSE against E. coli O157: H7 was 4.0 mg/ml. At high inoculation level (1 × 107 CFU/ml), including GSE at 0.25–2.0 mg/ml reduced Stx production without inhibiting E. coli O157:H7 growth. At 5 × 105 CFU/ml inoculation level, 2.0 and 4.0 mg/ml GSE effectively inhibited the growth of E. coli O157:H7 for at least 72 h, however, a low level of GSE (0.125–1.0 mg/ml) enhanced E. coli O157:H7 growth and Stx2 production. At 4 mg/ml, GSE completely abolished Stx2 production in addition to it bactericidal effect against E. coli O157:H7. In addition, GSE at concentration as low as 0.125% blocked the swimming motility, which is important for E. coli O157:H7 surface adherence. In conclusion, GSE is effective in inhibiting the motility of E. coli O157:H7, GSE shows potential to be used as a natural antimicrobial to control E. coli O157:H7.