Antimicrobial Efficacy of Contact Lens Solutions Assessed by ISO Standards.

Cindy Mcanally,Paul Shannon,Megan Thomas,Allison Campolo,Manal M Gabriel,Jamie King,Monica Crary,Rhonda Walters,Valerie Harris

doi:10.3390/microorganisms9102173

Abstract

Microbial keratitis (MK) is an eye infection caused by opportunistic bacteria or fungi, which may lead to sight-threatening corneal ulcers. These microorganisms can be introduced to the eye via improper contact lens usage or hygiene, or ineffective multipurpose solutions (MPSs) to disinfect daily wear contact lenses. Thus, the patient’s choice and use of these MPSs is a known risk factor for the development of MK. It is then critical to determine the efficacy of popular MPSs against ubiquitous ocular microorganisms. Therefore, we compare the efficacy of nine major MPSs on the global market against four different microorganism species, and with four different common contact lenses. In accordance with International Standards Organization protocol 14729 and 18259, the microorganisms were inoculated into each MPS with and without contact lenses, and held for the manufacturer’s disinfection time, 24 h, and 7 days after challenge with Serratia marcescens or Fusarium spp. Plates were incubated for 2–7 days and plate counts were conducted to determine the number of surviving microorganisms. The majority of MPSs demonstrated significantly higher disinfection efficacies without contact lenses. Broadly, among the microorganisms tested, the OPTI-FREE products (Puremoist, Express, and Replenish) maintained the highest disinfection efficacies at the manufacturer’s stated disinfection time when paired with any contact lens, compared with other MPSs. These were followed closely by RevitaLens and renu Advanced. MPSs containing dual biocides polyquaternium-1 and myristamidopropyl dimethylamine possessed the highest disinfection efficacy against multiple ocular pathogens.

Highlights

Microbial keratitis (MK), an infection resulting in corneal ulceration, is a sight-threatening complication affecting over 30,000 people in the United States per year [1]
Fusarium species have been found to be the causative microorganisms in a number of MK cases [7], as this opportunistic microorganism can be introduced to the eye via improper contact lens hygiene habits or ineffective biocides [8]
We have previously shown that commonly available Multipurpose solutions (MPSs) demonstrate strong biocidal efficacy against ocular pathogens such as Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans [13], previous investigations have yet to fully examine the more novel contact lenses available after challenge with resistant infectious microorganisms, such as the Fusarium and Serratia species

Summary

Introduction

Microbial keratitis (MK), an infection resulting in corneal ulceration, is a sight-threatening complication affecting over 30,000 people in the United States per year [1]. The differences in how well the biocides within each MPS are able to disinfect any particular contact lens are critical [1,2,3,4,5,6]. Within these MK outbreaks, Fusarium is a widely distributed genus of filamentous fungi that usually poses little danger to healthy individuals. Fusarium species have been found to be the causative microorganisms in a number of MK cases [7], as this opportunistic microorganism can be introduced to the eye via improper contact lens hygiene habits or ineffective biocides [8].

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