Abstract
Objective:The aim of the present in-vitro study was to assess antimicrobial efficacy of 0.8% hyaluronic acid (HA) and 0.2% Chlorhexidine gluconate (CHX) against Porphyromonas gingivalis (P. gingivalis).Methods:The study was performed between December 2018 and March 2019 at the College of Dentistry at the Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia. The P. gingivalis biofilms were formed and grown for 72 hours at 37°C under anaerobic conditions on glass slides coated with human saliva. The slides were individually positioned and exposed to 0.8% HA or 0.2% CHX. Therapeutically, the biofilms were divided into 3 groups as follows: (a) negative group; (b) 0.8% HA group and (c) 0.2% CHX group. P-values less than 0.05 were considered statistically significant.Results:In the 0.8% HA group, P. gingivalis CFUs/ml were significantly higher at baseline than at 24- (P<0.05), 48 (P<0.05) and 72 hours (P<0.05) intervals. In the 0.2% CHX group, P. gingivalis CFUs/ml were significantly higher at baseline than at 72 hours interval (P<0.05). In the CHX group, there was no difference in P. gingivalis CFUs/ml between baseline, 24- and 48-hours intervals. At 48- and 72-hours intervals, the P. gingivalis CFUs/ml were significantly higher in the 0.2% CHX group compared with the 0.8% HA group.Conclusion:In-vitro, 0.8% HA is more effective in reducing the P. gingivalis CFUs/ml compared with 0.2% CHX.
Highlights
Periodontitis is associated with inflammatory conditions including gingival inflammation, increased probing depth, clinical attachmentCorrespondence: July 20, 2019 August 1, 2019 October 12, 2019 October 16, 2019 loss; and resorption of supporting alveolar bone.[1]
In the 0.8% hyaluronic acid (HA) group, P. gingivalis Colony-forming units (CFUs)/ml were significantly higher at baseline compared with microbial colonization at 24- (P
In the 0.2% Chlorhexidine gluconate (CHX) group, P. gingivalis CFUs/ml were significantly higher at baseline compared with microbial CFUs/ ml at 72 hours interval (P
Summary
Periodontitis is associated with inflammatory conditions including gingival inflammation, increased probing depth, clinical attachmentCorrespondence: July 20, 2019 August 1, 2019 October 12, 2019 October 16, 2019 loss; and resorption of supporting alveolar bone.[1]. Periodontitis is associated with inflammatory conditions including gingival inflammation, increased probing depth, clinical attachment. Denticola), and Tannerella forsythia (T. forsythia), collectively known as Red Complex Bacteria (RCB).[3] The RCB are well-known microbes associated with the etiopathogenesis of periodontal inflammatory conditions, including periodontitis and triggers inflammatory signaling pathways thereby jeopardizing human gingival fibroblasts.[4]. HA commonly exists in the synovial fluid, cartilage, and tissues of the eye and skin.[5] The high molecular weight of HA exerts immunosuppressive and anti-inflammatory effects and promotes wound healing.[5] It has been reported that HA is present in the extracellular matrix of periodontal tissues and plays a role in maintaining a healthy periodontium.[6] In a clinical study, Vanden Bogaerde L.7 investigated the efficacy of esterified HA in the treatment of periodontitis. Chen et al.[8], proposed that HA has benefits periodontal tissues by reducing inflammation and its related parameters and promoting mound healing
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