Abstract
This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.
Highlights
Oropharyngeal candidiasis is an opportunistic infection of the mucosa caused by Candida species [1, 2] and Candida albicans is the most common etiological agent [3, 4]
For the extracts of leaves and fruits of C. saligna, even increasing the concentration to 0.015 g / mL, no efficacy was found against the cells of C. albicans, as well as the extracts obtained from the bark or trunk of the three species, which did not show antifungal activity at this concentration and were eliminated from the tests of this study
The null hypothesis of this study was rejected because the results showed that the extracts obtained from the leaves and fruits of C. moschata e C. mandiocana were able to reduce the number of colonies forming units and the cellular metabolism of the C. albicans biofilm formed on the acrylic resin samples, proving its antimicrobial effect
Summary
Oropharyngeal candidiasis is an opportunistic infection of the mucosa caused by Candida species [1, 2] and Candida albicans is the most common etiological agent [3, 4]. One such example of oropharyngeal infection is Denture Stomatitis (DS). Candida spp. infection, trauma, gender, nutritional deficiency, poor fitting dentures, continual wearing, reduced salivary flow, lack of hygiene are some of the factors involved in the DS etiology [8,9,10,11,12]. The presence of Candida ssp. biofilm remains one of the most important factors for the development of this condition
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