Antimicrobial effects of sodium hypochlorite and Er,Cr:YSGG laser against Enterococcus faecalis biofilm.

Abstract

The aim of this study was to investigate the antimicrobial effect of Erbium, chromium-doped yttrium, scandium, gallium, and garnet (Er,Cr:YSGG) laser with and without NaOCl solution. A total of 81 extracted human mandibular premolar teeth were used. After root canal preparation and sterilization, the samples were inoculated with E. faecalis for 24 hours. The specimens were divided into 4 experimental groups. Group 1 (n = 25) was irradiated with 2 W laser, group 2 (n = 25) was irradiated with 0.75 W laser in combination with 2.5% NaOCl, group 3 (n = 25) was irrigated with 5% NaOCl and group 4 (n = 6) was not treated. Statistical analysis was performed by using Wilcoxon Signed Ranks, Mann-Whitney U and Kruskal-Wallis H tests. The combination of 0.75 W laser with 2.5% NaOCl regime was found to be just as effective at inhibiting the growth of E. faecalis and sterilization of all root canals as 5% NaOCl irrigation (P > 0.001). The 2 W laser had significant bactericidal effect in infected root canals however it did not eradicate all bacteria. The SEM observations were in accordance with the microbiologic findings. Within the limitations of this laboratory study, NaOCl irrigation improved the antimicrobial effect of Er,Cr:YSGG laser irradiation. When the toxic effects of high percentage of NaOCl was considered, the combination of low-powered laser and low concentration of NaOCl can be used as an effective disinfection method in root canal treatment.