Antimicrobial Effects and Mechanisms of Ethanol Extracts of Psoralea corylifolia Seeds Against Listeria monocytogenes and Methicillin-Resistant Staphylococcus aureus.

Abstract

Psoralea corylifolia seeds contain many bioactive compounds commonly used in traditional Chinese medicine. In this study, the antibacterial activity and possible mechanism of P. corylifolia seed ethanol extract (PCEE) against foodborne pathogens were investigated. Both methicillin-resistant Staphylococcus aureus (MRSA) and Listeria monocytogenes had similar minimum inhibitory concentrations and minimum bactericidal concentrations of PCEE at 50 and 100 μg/mL, respectively. Furthermore, elevated OD260, protein concentration, and electric conductivity indicated irreversible damage to the cytoplasmic membranes of PCEE-treated cells. Indeed, the treated cells displayed disrupted membranes, incomplete and deformed shapes, and rupture as visualized by scanning electron microscopy. Multidrug-resistance efflux pump gene expression was also analyzed by quantitative reverse transcription PCR. Although the mdrL, mdrT, and lde genes of L. monocytogenes and the mepA gene of MRSA were upregulated, there was no significant difference that indicated an attempt by the efflux pumps to discharge PCEE. MRSA norA expression and abcA expression were significantly downregulated (p < 0.05). A possible mechanism for PCEE may be to cause an energy depletion, either by inhibiting adenosine triphosphate binding or by disturbing the proton gradient, resulting in membrane damage.