Antimicrobial effectiveness of grape seed extract against Enterococcus faecalis biofilm: A Confocal Laser Scanning Microscopy analysis.

Abstract

This study evaluated the antimicrobial effectiveness of 6.5% Vitis vinifera grape seed extract (GSE) against Enterococcus faecalis biofilm using confocal laser scanning microscopy (CLSM). Saline solution (SS), 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) were used for comparison. Dentin discs were inoculated with E.faecalis strain establishing a 3-week-old biofilm. Discs (n=10) were exposed to 5.25% NaOCl, 2% CHX, 6.5% GSE and SS (negative control) for 10min. Discs were stained with the fluorescent LIVE/DEAD-BacLight™ dye and analysed using CLSM. The proportion of dead cells in biofilm was analysed using one-way anova and Tukey tests (P<0.05). A higher proportion of dead cells was found in GSE group compared with CHX and SS (P<0.05). NaOCl group was associated with the highest proportion of dead cells (P<0.05). GSE presented antimicrobial activity against E.faecalis; however, NaOCl was the most effective irrigant solution. GSE was more effective than CHX and SS.