Abstract
One of the most important functions of skins is to protect our bodies from microbes or pollutant sources. Skins containing physical substances serve as a physical barrier which protects our bodies from pathogens. A healthy skin contains a variety of antibacterial substances such as defensin, cathelicidin and psoriasin. However deep and wide burns cause the skin to lose its original functions, so our skins are exposed to various danger factors. For the burn patients, human alloskin graft serves as a very important temporary biological wound dressing. It protects the wound before autograft procedure, forms revascularization and granulation tissues and protects the wound from an invasion of microbes. This study was conducted with the aim to analyze the antimicrobial effect of cryopreserved allograft (CPA) and glycerol-preserved allograft (GPA) which was a type of allograft widely used for burn patients, and measure the difference in comparison with the fresh skin before processing it. The most common contaminants found in burn patients such as S. aureus, P. aeruginosa, C. albicans and E. coli, were used for experiment. The antimicrobial effect against S. aureus and E. coli was observed in fresh skin and some CPA. In some clinical cases, infection is frequently observed in the wounds treated with allograft, indicating the allograft completely block every kind of microbes. To prevent the infection, it is required to use antibiotics and manage wounds thoroughly.
Highlights
This study was conducted with the aim to analyze the antimicrobial effect of cryopreserved allograft (CPA) and glycerol-preserved allograft (GPA) which was a type of allograft widely used for burn patients, and measure the difference in comparison with the fresh skin before processing it
The results were no bacteria in fresh skin, CPA and GPA samples (Table 1)
Ekrami and Lalantar verified throughout their study that 140 of 180 burn patients were exposed to a risk of infection especially by pathogenic bacteria such as P. aeruginosa (37.5%), S. aureus (20.2%) and A. baumanni (10.4%) [16]
Summary
Skin consisting of numerous cells controls the immune response and serves. K. Lim et al 2 as a primary protective layer [1]. Skin protects our body from invasion of microbes by way of serving as a barrier and discharging a wide range of chemical substances [2] [3]. Skin discharges more than 20 different types of AMP in the event of infection. It is widely known that skin secretes antibiotics such as cathelicidin, defensin, regulated on activation, normal T cell expressed and secreted (RANTES) reported that psoriasin secreted from keratinocyte showed bactericidal capacity against E. coli [4] [5]. If skins are burned, dermis and epidermis are destroyed, resulting in inflammation, the malfunction of immune system and infection [6]
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