Abstract

Purpose: To investigate the effect of silver ionised water on acid production in plaque. Methods: After injecting 0.5 mL of silver ionised water (concentration: 5 ppm) produced with the sintering coating method in the sensor part of the pH metre, plaque collected from the oral cavity by one platinum loop was mixed in. Immediately after that, a 5% sucrose solution (1 g/20 mL) was added and the pH was continuously measured for 30 minutes at 1-minute intervals (A). Similarly, silver ionised water was mixed with 5% sucrose solution in the same way as in (A) at 3 (B), 5 (C), 10 (D) and 15 minutes (E) after plaque contamination. The pH was measured at 1-minute intervals. The pH of the purified water containing no silver ionised water mixed with plaque and sugar solution at the same time was measured and used as a control. Each experiment was conducted three times, and the pH measured every minute was compared as a percentage of the pH at the beginning of the measurement (100%). Results: Analysis of variance of the repeated measurements to determine the effect of silver ionised water on the decrease in pH revealed a main effect of silver ionised water and an interaction between time and group [F(1.302,20.826) = 39.145, p < 0.01]. Multiple comparisons using Dunnett’s method showed a significant decline in the rate of decrease in pH from B to E as compared with that in the control (p < 0.01). Conclusion: Silver ionised water was found to inhibit the acid production in plaque.

Highlights

  • Silver is a metal that has been known for a long time

  • We investigated the effect of silver ionised water on the acid production of plaque for application in clinical dentistry

  • Sugar solution of the same concentration was added to the silver ionised water for 3 (B), 5 (C), 10 (D), and 15 minutes (E) after plaque contamination and the pH was measured for 30 minutes at 1-minute intervals

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Summary

Introduction

Silver is a metal that has been known for a long time. Silver has long been known to have strong antibacterial [1] [2] [3] and antifungal properties [4]. As an effect on plaque, the rate of plaque adhesion was reported to be suppressed after mouth washing with silver-ion water [10]. In addition to tooth brushing, mouthwash can be used as a sanitising method, but no studies have been conducted to clarify the effect of silver ionised water on plaque on teeth. We investigated the effect of silver ionised water on the acid production of plaque for application in clinical dentistry

Preparation of Silver-Ion Water
Experimental Method
Statistical Analysis
Results
Silver Ionised Water
Analysis of the Results
Antimicrobial Mechanism of Silver Ions
Effect on Acid Production in Plaque
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