Antimicrobial effect of chlorhexidine on Aggregatibacter actinomycetemcomitans biofilms associated with peri-implantitis.

Zeinab Kadkhoda,Nazanin Samiei,Saeed Eshraghi,Zeinab Amarlu

doi:10.15171/joddd.2016.028

Abstract

Background. This study aimed to assessthe antimicrobial effect of chlorhexidine (CHX) on Aggregatibacter actinomycetemcomitans biofilms isolated from subgingival plaque of peri-implantitis lesions.Methods. Thirteen patients requiring peri-implantitis treatment were consecutively selected and their subgingival biofilm was collected by inserting fine sterile paper points into peri-implant pockets for 15 seconds. A. actinomycetemcomitans was isolated from the subgingival biofilm and cultured. In this study, the standard strain of A. actinomycetemcomitans served as the positive control group and a blank disc impregnated with water served as the negative control; 0.1 mL of the bacterial suspension was cultured on specific culture medium and blank discs (6 mm in diameter) impregnated with 0.2%CHX mouthrinse (Behsa Pharmaceutical Co.) and negative control discs were placed on two sides of the bacterial culture plate. The size of growth inhibition zone was measured by a blinded independent observer in millimetres.Results. According to the results of disc diffusion test, the mean diameter of growth inhibition zone of A. actinomycetemcomitans around discs impregnated with CHX was larger in both standard (positive control) and biofilm samples of A. actinomycetemcomitans compared to the negative control group (blank disc) (P<0.001).Conclusion. Use of0.2% CHX mouthwash had antibacterial effects on A. actinomycetemcomitans species isolated from peri-implantitis sites.

Highlights

Peri-implantitis is defined as a destructive inflammatory process of the peri-implant soft and hard tissues, often resulting in loss of supporting bone structure beyond biological bone remodelling.[1,2] Reports are variable regarding the JODDD, Vol 10, No 3 Summer 2016 incidence and prevalence of peri-implantitis and a prevalence of 6.61% over a 9‒14-year period,3 23% during a 10-year period[4] and 36.6% during an average of 8.4 years of loading.[5]
This study aimed to assessthe antimicrobial effect of chlorhexidine (CHX) on Aggregatibacter actinomycetemcomitans biofilms isolated from subgingival plaque of peri-implantitis lesions
Use of0.2% CHX mouthwash had antibacterial effects on A. actinomycetemcomitans species isolated from peri-implantitis sites

Summary

Introduction

Peri-implantitis is defined as a destructive inflammatory process of the peri-implant soft and hard tissues, often resulting in loss of supporting bone structure beyond biological bone remodelling.[1,2] Reports are variable regarding the JODDD, Vol 10, No 3 Summer 2016 incidence and prevalence of peri-implantitis and a prevalence of 6.61% over a 9‒14-year period,3 23% during a 10-year period[4] and 36.6% during an average of 8.4 years of loading.[5] The specific role of bacteria in peri-implantitis was argued recently. This study aimed to assessthe antimicrobial effect of chlorhexidine (CHX) on Aggregatibacter actinomycetemcomitans biofilms isolated from subgingival plaque of peri-implantitis lesions

Objectives

Methods

Results

Conclusion