Antimicrobial capacity of Leucocyte-and Platelet Rich Fibrin against periodontal pathogens

Ana B Castro,Wim Teughels,Nelson Pinto,Esteban R Herrero,Marc Quirynen,Vera Slomka

doi:10.1038/s41598-019-44755-6

Ana B Castro, Wim Teughels + Show 4 more

Open Access

https://doi.org/10.1038/s41598-019-44755-6

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Journal: Scientific Reports	Publication Date: Jun 3, 2019
Citations: 50	License type: open-access

Affiliation: KU Leuven, Universidad de Los Andes, Chile

Abstract

Various studies have described the biological properties of the Leucocyte- and Platelet Rich Fibrin (L-PRF) such as the antimicrobial effect against wound bacteria, but less is known about the effect against periodontal pathogens. The aim of this study was to evaluate the antibacterial properties of the L-PRF membrane and L-PRF exudate against the main periopathogens cultured on agar plates and in planktonic solution. This study demonstrated the antibacterial effect of the L-PRF membrane against P. intermedia, F. nucleatum, and A. actinomycetemcomitans, but especially against P. gingivalis. The L-PRF exudate also showed a strong inhibition against P. gingivalis on agar plates. No inhibition could be observed for the other bacterial strains. Moreover, L-PRF exudate decreased the number of viable P.gingivalis in a planktonic solution in a dose-dependent way. However, A. actinomycetemcomitans showed an increased growth in planktonic solution when in contact with the L-PRF exudate.

Highlights

The classic experimental gingivitis studies in the 60 s1,2 demonstrated the direct relation between the accumulation of dental plaque and gingival inflammation
Given the cellular composition of L-PRF16 and its bioactive nature[17], the aim of this study was to evaluate the antimicrobial capacity of an Leucocyte- and platelet-rich fibrin (L-PRF) membrane and L-PRF exudate against key periodontal pathogens (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans)
The results in this study demonstrated antimicrobial activity of the L-PRF membrane against P. gingivalis

Summary

Introduction

The classic experimental gingivitis studies in the 60 s1,2 demonstrated the direct relation between the accumulation of dental plaque and gingival inflammation. Studies on biofilm development in deep periodontal pockets showed that the deepest sites are colonized predominantly by motile species (e.g. spirochetes) and gram-negative bacteria, located adjacent to the epithelial lining of the pocket. Some of these bacteria are part of the red complex (Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia) and the orange complex (e.g. Fusobacterium nucleatum)[4]. L-PRF is prepared from the patient’s own blood, without adding any additives, and concentrates >90% of the platelets and >75% of the leucocytes from the initial blood composition[16] It offers a continuous release of growth factors and other bioactive substances that stimulate and protect the surgical site[17,18]. Inhibit the four tested bacterial strains when applied directly on a cultured Brain Heart Infusion (BHI) agar plate, and (2), L-PRF exudate does not inhibit bacterial growth of the tested bacterial strains in planktonic form

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Conclusion