Abstract
Antimicrobial biopolymers provide a biodegradable, sustainable, safe, and cheap approach to drug delivery and wound dressing to control bacterial infection and improve wound healing respectively. Here, we report a one-step method of making antimicrobial alginate polymer from sodium alginate and aqueous extract of Wakame using antibiotic aminoglycosides. Thin layer chromatography of commercially available sodium alginate and Wakame extract showed similar oligosaccharide profiles. Screening of six aminoglycosides showed that kanamycin disulfate and neomycin sulfate produces the highest amount of biopolymer; however, kanamycin disulfate produces the most malleable and form fitting biopolymer. Image texture analysis of biopolymers showed similar quantification parameters for all the six aminoglycosides. Weight of alginate polymer as a function of aminoglycoside concentration follows a growth model of prion protein, consistent with the aggregating nature of both processes. Slow release of antibiotics and the resulting zone of inhibition against E. coli DH5α were observed by agar well diffusion assay. Inexpensive method of production and slow release of antibiotics will enable diverse applications of antimicrobial alginate biopolymer reported in this paper.
Highlights
According to the World Health Organization (WHO), the emergence of multidrug resistance among bacterial pathogens is a global public-health challenge [1]
We selected kanamycin disulfate (KDS) for further testing because it is relatively inexpensive and made alginate biopolymer from sodium alginate (SA) and Wakame extract, both of which resulted in good quality polymer (Fig 1f and 1g); SA solution provided more malleable (Fig 1f, left panel) and form fitting (Fig 1g, right panel) polymer
We considered the cost of aminoglycosides and the amount of alginate polymer produced using 1 mg of aminoglycoside to determine the efficiency of polymerization, which follows the sequence gentamicin sulfate (GS)>neomycin sulfate (NS)>streptomycin sulfate (SS)>tobramycin sulfate (TS)>kanamycin sulfate (KS)>KDS in decreasing order of efficiency
Summary
According to the World Health Organization (WHO), the emergence of multidrug resistance among bacterial pathogens is a global public-health challenge [1]. WHO has listed Acinetobacter baumannii [2, 3], carbapenem-resistant Pseudomonas aeruginosa [4, 5], and carbapenem-resistant Enterobacteriaceae [6, 7] as critical priority pathogens These pathogens are responsible for infections of burn, wound, blood stream, nervous system, urinary and respiratory tracts. In this context, antimicrobial biopolymer synthesis is one of the current areas of antimicrobial drug delivery research to control bacterial infection in biomedical devices [8], wound healing [9], food packaging [10], textiles [11], cosmetic products [11], and water treatment systems [12]. While some polymers have intrinsic antimicrobial activity [14], others have antimicrobial compounds
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
