Abstract
The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p <0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.
Highlights
The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, little research has documented about its biological potential and phytochemical profile
The following yields were obtained from the preparation of E. involucrata plant extracts with different solvents: MeOH (23.6%), EtOH (19.42%), Ace (9.38%), ethyl acetate (EtAce) (6%), Hex (6.35%), DCM (6.31%) and distilled water (DW) (9.84%)
BHT, methanol (MeOH), ethanol (EtOH), ethyl acetate (EtAce), acetone (Ace), hexane (Hex), dichloromethane (DCM) and distilled water (DW); Values of IC50 (Concentration of E. involucrata leaves extract is necessary to reduce 50% of the DPPH radical) expressed as mean ± standard deviation; Different letters in the same column express significant differences (p
Summary
The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, little research has documented about its biological potential and phytochemical profile. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). A partir das folhas de E. involucrata, foram obtidos extratos aquosos e orgânicos com os seguintes solventes (etanol, metanol, hexano, acetona, diclorometano e acetato de etila). As one of the places with the highest abundance of plant biodiversity, has become a country of great origin in the search for biologically active principles, supplying raw materials.
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