Abstract
Dialium angolense is used in Bagira for its various medicinal properties particularly in the management of infectious diseases. In this study, the methanol and aqueous extracts of leaves and fruits were evaluated for their in vitro antioxidant and antimicrobial properties and their in vivo toxicity on Cavia porcellus. The major phytochemical classes of extracts were screened using standard in-tube reactions. The antimicrobial study was tested on Candida albicans, Escherichia coli, Salmonella typhi, Staphylococcus aureus and Streptococcus pneumoniae using agar well diffusion and dilution methods, while the antioxidant activity was evaluated by a DPPH assay. For the acute toxicity study, animals (6/group) were orally given in a single dose 5000, 1000 or 15000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assays, 150 or 300 mg/kg BW/day were orally given, and animals observed for 28 days. Total phenolics and total flavonoids contents ranged 1.19 to 1.61 mg GAE.g-1 and 0.45 to 1.01 mg QEg-1, respectively. The extracts presented antioxidant activity with IC50 ranging 4.9 to 6.9 µg/mL. The minimal inhibitory concentration (MIC) on tested strains ranged from 1.9 to 500 µg/mL with the aqueous extract of fruits as a most active extract: MIC=1.9 µg/mL on E. coli and C. albicans. No signs of toxicity were noted during the acute and sub-acute toxicity assessments, suggesting a maximal tolerate doses (MDT) and LD50 > 15000 mg/kg BW. This study highlights the antioxidant and antimicrobial activities of Dialium angolense and suggests that further studies be directed towards the isolation of active compounds.
Highlights
Microbial infections are a major health concern because of their high prevalence and of their high mortality in most African countries like the DR Congo [1,2,3,4]
The antimicrobial study was tested on Candida albicans, Escherichia coli, Salmonella typhi, Staphylococcus aureus and Streptococcus pneumoniae using agar well diffusion and dilution methods, while the antioxidant activity was evaluated by a diphenyl-2- picrylhydrazyl radical (DPPH) assay
Chemical screening of Dialium angolense The phytochemical screening of Dialium angolense revealed the presence of polyphenols, tannins, terpenoids, flavonoids, coumarins, anthraquinones but the absence of alkaloids and saponins (Table 1)
Summary
Microbial infections are a major health concern because of their high prevalence and of their high mortality in most African countries like the DR Congo [1,2,3,4]. There is currently a tendency to investigate antimicrobial and antioxidant activities as shown by several recent studies carried out on African medicinal plants [18,19,20]. Ex Oliv., called Dialium evrardii (Steyaert) belongs to the Fabaceae family, with many local names like Kizimya (Shi) or Cituzo (Havu); is one of those medicinal plants that are not well known. A recent study reported antioxidant and antiplasmodial activities in vitro of aqueous and methanolic extracts of Dialium angolense leaves [25]. This study aims to evaluate the antimicrobial activity of aqueous and methanolic extracts of leaves and fruits of Dialium angolense on germs responsible for some digestive, respiratory and nosocomial infections. It intends to establish its phytochemical profile in secondary metabolites and assess the acute and subacute toxicity of its two organs, on Cavia porcellus
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
