Antimicrobial, antibiofilm, and antioxidant activity of chitosan nanoparticles synthesized by E. coli

Abstract

The aim of the this study was to study the antimicrobial, antibiofilm, and antioxidant activity of Chitosan nanoparticles synthesized by E. coli. Twenty five Sample and investigations were achieved during the period from November 2018 to April 2019. Wound infection samples were collected from Two different hospital in Najaf province (AL-Sadder medical city). The A5 (E.coli) efficient isolate. the isolate was diagnosed by convenatial and molecular methods. Molecular study showed the E,coli isolate gave positive results (1270bp) for 16s rRNA assay, Data of E. coli strain at the locus (MN197856), accession (MN197856), version (MN197856.1), defined as (Escherichia coli strain Kafeel-1 16S ribosomal RNA gene, partial sequence. The results showed the gram positive bacteria highly resistante to novobiocin and nitrofurantion with the diameter of inhibition zone 27 and 20mm respectively, and low resistante to amoxicillin/calvulanic acid with the diameter of inhibition zone (0 mm). Where gram negative bacteria showed highly resistante to imipenem with the diameter of inhibition zone 22 mm. and low resistante to antibiotic includes ciprofloxacin and amoxicillin/calvulanic acid with the diameter of inhibition zone 5 and 0 mm respectively. Biogenic chitosan expressed high significant antibiofilm activity with increasing concentration of chitosan NPs. DPPH reducing activity of nanoparticles which increased with increase concentration of biogenic chitosan NPs.