Abstract
Hemp (Cannabis sativa L.), a medicinal plant of family Cannabaceae, growing wildly in most of the rainfed areas of Punjab, Pakistan. In this study, phytochemical profile of methanolic extract of root was assessed through GC-MS analysis and various biologically active compounds were identified through literature survey. There were 14 compounds in the root extract. The most abundant compounds were γ-sitosterol (27.08%) and 9,12-octadecadienoic acid (Z,Z)-, methyl ester (24.09%) and hexadecanoic acid, methyl ester (21.81%). Other identified compounds were methyl stearate (5.51%), stigmasterol (5.12%), campesterol (4.19%), phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl-(2.52%), 11-octadecenoic acid, methyl ester (2.10%), squalene (1.89%), β-amyrin (1.88%), eicosanoic acid, methyl ester (0.85%), tetracosanoic acid, methyl ester (0.88%), dronabinol (1.02%) and 2-methoxy-4-vinylphenol (0.96%). Most of the identified compounds possess one or more biological activities viz. antitumor, antifungal, antibacterial, antioxidant, anticancer, anti-inflammatory, antidiabetic, and/or analgesic.
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