Abstract
Objective: Our study was to isolate Bacillus spp. from soil all around 6 geographic parts of Thailand and screen for potential antimicrobial.Methods: A total of 43 isolates which isolated from 100 samples of soil were investigated. Preliminary screening was based on antimicrobial activity against 16 strains of pathogenic bacteria and fungi, including 10 strains of Methicillin-resistant Staphylococcus aureus (MRSA), and 1 strain of Methicillin-sensitivity Staphylococcus aureus (MSSA), Extended spectrum beta lactamases (ESBL) Escherichia coli, Samonella Typhymurium, Klebsiella pneumoniae, Candida albicans, Cryptococcus neoformans respectively by cross streak, agar diffusion and modified microdilution technique. Moreover, the selected Bacillus spp. were then screened for bioactive enzyme, including chitinese, chitosanese, amylase, cellulose, caseinase, gelatinase, esterase and lipase production of different selective media for 24 and 48 h by direct spot agar.Results: There are 2 isolates, namely 23 and 49 showed particularly strong activity inhibitions against MRSA and pathogenic C. albicans. The diameter of hydrolysis zone results from screened for bioactive enzyme revealed that there were 36 isolate showed particularly strong activities with a broad spectrum enzyme. The isolate which produced the widest diameter hydrolysis zone in gelatin, cellulose and starch are 294, 303, and 290 respectively.Conclusion: Our result indicated Bacillus spp. from soil in Thailand showed potent antibacterial activity and bioactive compounds production.
Highlights
The uses of antibiotics are widespread in clinical medicine, agriculture, aquaculture, and veterinary promote the development of antibiotic resistances in infectious microbial strains and eventually reflects a very serious problem in the treatment of pathogenic microbes
We described a preliminary characterization of Bacillus species isolated from soil of Thailand and to assess their antimicrobial activity as well as enzyme production capacity based on molecular methods
The present study was pointing out to Bacillus spp. due to their wide ubiquity in nature and genetic and metabolic diversity leading the production of several antibiotics and enzymes, these bacteria have become increasingly interesting for different biotechnological applications nowadays
Summary
The uses of antibiotics are widespread in clinical medicine, agriculture, aquaculture, and veterinary promote the development of antibiotic resistances in infectious microbial strains and eventually reflects a very serious problem in the treatment of pathogenic microbes. It is necessary to search for novel antibiotics through natural, synthetic or semisynthetic sources. Microorganisms are the most potential source for production of natural therapeutic agents. Searches of bioactive compound from microorganisms are considered for developing the therapeutic drugs, which are highly effective, low toxic and having minor environmental impact. In the course of screening for new antibiotics, several research studies are currently oriented towards isolation of new microorganism species from different soils and ecosystems [4, 5]
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