Antimicrobial and Antioxidative Activity of Newly Synthesized Peptides Absorbed into Bacterial Cellulose Carrier against Acne vulgaris.

Iwona Golonka,Katarzyna E Greber,Monika Oleksy-Wawrzyniak,Andrzej Dryś,Justyna Paleczny,Witold Musiał,Adam Junka,Wiesław Sawicki

doi:10.3390/ijms22147466

Abstract

The ongoing search for effective treatment of Acne vulgaris is concentrated, i.a., on natural peptides with antimicrobial properties. The aim of this work was the development of new amino acid derivatives with potential activity on dermal infections against selected microorganisms, including the facultative anaerobe C. acne. The peptides P1–P6 were synthesized via Fmoc solid phase peptide synthesis using Rink amide AM resin, analyzed by RP-HPLC-MS, FTIR, DPPH radical scavenging activity, and evaluated against C. acne and S. aureus, both deposited and non-deposited in BC. Peptides P1–P6 presented a lack of cytotoxicity, antimicrobial activity, or antioxidative properties correlated with selected structural properties. P2 and P4–P6 sorption in BC resulted in variable data, i.a., confirming the prospective topical application of these peptides in a BC carrier.

Highlights

Acne vulgaris belongs to dermatoses of complex origin, engaging interactions between microbiological, genetic, dietary, immunological, hormonal, and environmental factors [1]
In our own studies we have found, and had confirmed by other researchers, that bacterial cellulose (BC) may be used for the absorption of numerous substances, while its biological activity is maintained after such procedures [22]
The antioxidant properties of the tested compounds were influenced by amino acid residues such as tryptophan, lysine, and arginine in the peptide structure, which increased the antioxidant properties of the compound

Summary

Introduction

Acne vulgaris belongs to dermatoses of complex origin, engaging interactions between microbiological, genetic, dietary, immunological, hormonal, and environmental factors [1] It affects an increasing number of patients, and is diagnosed mainly within young populations; in adults the manifestation of acne may become acute and present with severe inflammatory lesions. One of the microbiological factors in its development is the excessive growth of the facultative anaerobe, Cutibacterium acnes, formerly described as Propionibacterium acnes, a species naturally occupying the skin’s surface and hair follicles This leads to an increased release of pro-inflammatory compounds, resulting in immoderate activation of immune system components [2,3,4,5,6,7,8]. We propose a method of replacing the missing components of the altered epidermis and stimulating the production of endogenic substances [18]

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Conclusion