Abstract
Pycnocycla spinosa (P. spinosa) a member of the Umbelliferae family is traditionally used for treatment of different ailments. This study aimed to evaluate the total phenolic and flavonoid content of P. spinosa extracts (methanol, ethanol and aqueous) and their antioxidant and antimicrobial effects. The antimicrobial activity of different extracts of P. spinosa was evaluated using micro broth dilution. Total phenolic and flavonoid contents were measured. Their antioxidant effect was evaluated using DPPH assay and β-carotene linoleic acid test. P. spinosa ethanol extract with higher-level phenolic and flavonoid contents showed the highest antioxidant and antimicrobial effects, in comparison with the other extracts. Bacillus sp. and Streptococcus sp. showed higher sensitivity to P. spinosa ethanol extract. P. spinosa ethanol extract can be used as a mouthwash for treatment of the oral infections. More clinical and toxicological studies are required for providing its efficacy.
Highlights
Pycnocycla spinosa (P. spinosa) a member of the Umbelliferae family is traditionally used for treatment of different ailments
The P. spinosa aqueous extract had a lower total phenolic and flavonoid contents compared to the other extracts (33.9 and 3.8 mg/g)
In the DPPH assay, the P. spinosa ethanol extract IC50was lower than the other extracts (82 μg/mL) but was higher than the IC50 for BHT (18 μg/mL)
Summary
Pycnocycla spinosa (P. spinosa) a member of the Umbelliferae family is traditionally used for treatment of different ailments. Objectives: This study aimed to evaluate the total phenolic and flavonoid content of P. spinosa extracts (methanol, ethanol and aqueous) and their antioxidant and antimicrobial effects. Materials and Methods: The antimicrobial activity of different extracts of P. spinosa was evaluated using micro broth dilution. Total phenolic and flavonoid contents were measured Their antioxidant effect was evaluated using DPPH assay and β-carotene linoleic acid test. Results: P. spinosa ethanol extract with higher-level phenolic and flavonoid contents showed the highest antioxidant and antimicrobial effects, in comparison with the other extracts. Antibiotic-resistant microorganisms and side effects of these antibiotics concern the scientists They have been encouraged to find new sources of antimicrobial agents, especially among the medicinal plants. Elemicin (65%), linalyl acetate (11%), β-caryophyllene (7%) and β-eudesmol (4%) were the main constituents of P. spinosa seed essential oil [6]
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