Antimicrobial and Antioxidant Activities of Coumarins from the Roots of Ferulago campestris (Apiaceae)

Adriana Basile,Vivienne Spadaro,Maurizio Bruno,Sergio Sorbo,Antonella Maggio,Nicoletta Faraone,Sergio Rosselli

doi:10.3390/molecules14030939

Adriana Basile, Vivienne Spadaro + Show 5 more

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https://doi.org/10.3390/molecules14030939

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Journal: Molecules	Publication Date: Feb 27, 2009
Citations: 219	License type: CC BY 4.0

Affiliation: University of Palermo

Abstract

We report the isolation of several coumarins and the stereochemical assessment of some pyranocoumarins, as well as the antibacterial and antioxidant activities of the three most abundant ones (grandivittin, agasyllin and aegelinol benzoate) isolated from the roots of Ferulago campestris collected in Sicily and of the hydrolysis product (aegelinol). Aegelinol and agasyllin showed antibacterial activity against nine ATCC and the same clinically isolated Gram-positive and Gram-negative bacterial strains. At a concentration between 16 and 125 μg/mL both coumarins showed a significant antibacterial effect against both Gram-negative and Gram-positive bacteria. In particular the ATCC strains Staphylococcus aureus, Salmonella thypii, Enterobacter cloacae and Enterobacter earogenes (MIC = 16 and 32 μg/mL for aegelinol and agasyllin, respectively) were the most inhibited. Antibacterial activity was also found against Helicobacter pylori: a dose-dependent inhibition was shown between 5 and 25 μg/mL. The antioxidant activity of the coumarins was evaluated by their effects on human whole blood leukocytes (WB) and on isolated polymorphonucleate (PMN) chemiluminescence (CL), PMA-stimulated and resting.

Highlights

Ferulago campestris (Besser) Grec., (F. galbanifera (Mill) Kock. = Ferula ferulago L.), finocchiazzo, is an annual or perennial herb with small flowers that grows in the Mediterranean area.Previous phytochemical studies on the roots of F. campestris collected in Egypt revealed the presence of monoterpene coumarins and sesquiterpene lactones [1]
The antioxidant activity of the coumarins was evaluated by their effects on human whole blood leukocytes (WB) and on Molecules 2009, 14 isolated polymorphonucleate (PMN) chemiluminescence (CL), phorbol myristate acetate (PMA)-stimulated and resting
In continuation of our research for biologically active compounds from Sicilian medicinal plant sources [4,5], we investigated the constituents of the roots of Ferulago campestris

Summary

Introduction

Aegelinol and agasyllin showed antibacterial activity against nine ATCC and the same clinically isolated Gram-positive and Gram-negative bacterial strains. At a concentration between 16 and 125 μg/mL both coumarins showed a significant antibacterial effect against both Gram-negative and Gram-positive bacteria. The antioxidant activity of the coumarins was evaluated by their effects on human whole blood leukocytes (WB) and on Molecules 2009, 14 isolated polymorphonucleate (PMN) chemiluminescence (CL), PMA-stimulated and resting.

Results

Conclusion