Abstract
In this study we investigated the antimicrobial and anticancer activities of ethyl acetate extract of co-culture of Streptomyces sp. ANAM-5 and AIAH-10 isolated from soil of mangrove forest Sundarbans, Bangladesh. The antimicrobial activity of ethyl acetate extract was determined using broth-dilution method against Candida albicans, Saccharromyces cerevaceae and Aspergillus niger whereas anticancer activity was evaluated against Ehrlich Ascites Carcinoma (EAC) cells in Swiss albino mice with the dose of 50 mg/kg and 100 mg/kg body weight (i.p). The Minimum Inhibitory Concentrations (MIC) of ethyl acetate extract was found 32μg/ml against Candida albicans while 64 μg/ml against Saccharromyces cerevaceae and Aspergillus niger. The antineoplastic activity of the crude extract was increased in dose dependent manner with a significant value (p<0.01). Bacterial crude extract enhanced the mean survival time (MST) of tumor bearing mice at 71.79% and maximum cell growth inhibition was found 75.75 % with dose of 100 mg/kg body weight (i.p.). Our study revealed that ethyl acetate extract of co-culture of Streptomyces sp. ANAM-5 and AIAH-10 is an excellent source of antimicrobial and anticancer compounds which may become helpful to treat infections and cancer.
Highlights
Since the ancient time people utilize different natural sources for the cure of diseases
The antimicrobial activity of ethyl acetate extract was determined using broth-dilution method against Candida albicans, Saccharromyces cerevaceae and Aspergillus niger whereas anticancer activity was evaluated against Ehrlich Ascites Carcinoma (EAC) cells in Swiss albino mice with the dose of 50 mg/kg and 100 mg/kg body weight (i.p)
We report the minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and anticancer activity of a crude ethyl acetate extract obtained from the mixed fermentation of two marine Streptomyces sp
Summary
Since the ancient time people utilize different natural sources for the cure of diseases. Among such natural sources microbial secondary metabolites have been considered one of the powerful resources for drug discovery owing to their diverse biological activities. The marine environment could be an interesting source of the rare bacterial groups and biologically active molecules (Asha et al, 2006; Munro et al, 1999; Pelaez, 2006; Pomponi, 1999). The bioactive molecules produced by marine microorganisms have more novel and unique structures due to their complex living environment and diversity of species (Carte, 1996; Schwartsmann et al, 2001). Recent reports by some scientists have revealed a new dimension in producing new bio-active compounds by co-culturing microorganisms. The genomic analysis of some Streptomyces strains revealed the presence of biosynthetic gene clusters for about 30 secondary metabolites (Bentley et al, 2002; Ikeda et al, 2003; Ohnishi et al, 2008)
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