Abstract
The biosurfactants represent a class of biocompounds of growing interest for biomedical applications. The yeast strain Rhodotorula glutinis CMGB-RG1 assimilated normal dodecane (C12H26) with high rates, producing biosurfactants with constant emulsification index (29%) after two, respectively, three days. Best antimicrobial activity was recorded for the [50X] concentrated biosurfactant against Candida krusei CMGB-Y8 from vaginal infections. The crude biosurfactant showed high microbial inhibition of biofilms formed by Candida krusei CMGB 94 (35%), while for C. krusei CMGB-Y8 best results were obtained with [50X] concentrated biosurfactant (47%) due to the presence of more effective mechanism for biofilm formation and adhesion. The results represent a basis for further studies regarding the characterization of antimicrobial mechanism of action of R. glutinis biosurfactants against a large range of human pathogenic microorganisms.
Highlights
The yeast Candida albicans is the most prevalent cause of candidemia
Our present work focuses on the ability of the strain Rhotorula glutinis CMGB-RG1 to assimilate n-dodecane producing biosurfactants with antimicrobial and antiadhesion abilities
Biodegradation assay In order to assess n-dodecane biodegradation, the strain R. glutinis CMGB-RG1 was grown for 20 hours at 28 oC, 150 rpm on Yeast Peptone Glucose medium with Glycerol (YPG) medium, and 5 mL of culture were centrifuged for 6 min at 6000 rpm
Summary
The yeast Candida albicans is the most prevalent cause of candidemia. it seems that other Candida species such as C. parapsilosis, C. tropicalis, C. glabrata and C. krusei have an important role in causing non-albicans candidemia [1, 2]. Pathogenic Candida species adhere to medical devices and subsequently form biofilms with extremely negative impact on human health [3, 4]. Microbial biosurfactants represent a modern approach in controlling the proliferation of pathogenic microorganisms and fighting against biofilm formation [5] due to their compatibility with human hosts and to their high stability and specificity [6]. Many yeast species are good biosurfactants producers, Rhodotorula glutinis being able to synthesize carbohydrate-protein complexes [7] or polyol lipids [8] by degrading diesel, alkanes, phenol or industrial wastes [9-11]. Some n-alkanes, such as n-dodecane, can be found in various plant oils with high antimicrobial and antioxidant activities [12] and are used as excipients in drug industry [13]. Identification of new yeast strains able to assimilate n-dodecane and to produce biocompounds with different practical applications, represent an interesting research domain
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