Abstract
C. coli from slaughtered pigs and from human patients were examined for resistance to quinolones and tetracycline. Detection of tetO was performed. Gyrase A gene (gyrA) was amplified and sequenced and tested by an alternative method. Tetracycline resistance levels were respectively of 67,7 % and 56,3 % in swine and human isolates. In C. coli of human origin, all resistant isolates had the tetO determinant while 82,8 % of resistant C. coli from swine possessed it. Among the susceptible swine isolates, 17,2 % possessed this gene. Resistance to enrofloxacin (7,3 %) and ciprofloxacin (11,4 %) was observed in swine isolates and resistance for enrofloxacin (12,5 %) and ciprofloxacin (18,8 %) were observed in C. coli from humans. In addition, 72,7 % of swine resistant isolates and all isolates from humans had a mutation at position 86. Results were similar with MAMA-PCR which can thus be considered as a good alternative to sequencing.
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