Antimicrobial activity of zinc and titanium dioxide nanoparticles against biofilm-producing methicillin-resistant Staphylococcus aureus

A Jesline,P M Narayanan,Sevanan Murugan,C Vani,Neetu P John

doi:10.1007/s13204-014-0301-x

A Jesline, P M Narayanan + Show 3 more

Open Access

https://doi.org/10.1007/s13204-014-0301-x

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Journal: Applied nanoscience	Publication Date: Mar 13, 2014
Citations: 185	License type: CC BY 4.0

Affiliation: Karunya University

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major nosocomial pathogens responsible for a wide spectrum of infections and the emergence of bacterial resistance to antibiotics has lead to treatment drawbacks towards large number of drugs. Formation of biofilms is the main contributing factor to antibiotic resistance. The development of reliable processes for the synthesis of zinc oxide nanoparticles is an important aspect of nanotechnology today. Zinc oxide and titanium dioxide nanoparticles comprise well-known inhibitory and bactericidal effects. Emergence of antimicrobial resistance by pathogenic bacteria is a major health problem in recent years. This study was designed to determine the efficacy of zinc and titanium dioxide nanoparticles against biofilm producing methicillin-resistant S. aureus. Biofilm production was detected by tissue culture plate method. Out of 30 MRSA isolates, 22 isolates showed strong biofilm production and 2 showed weak and moderate biofilm formation. Two strong and weak biofilm-producing methicillin-resistant S. aureus isolates were subjected to antimicrobial activity using commercially available zinc and titanium dioxide nanoparticles. Thus, the nanoparticles showed considerably good activity against the isolates, and it can be concluded that they may act as promising, antibacterial agents in the coming years.

Highlights

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major nosocomial pathogens responsible for a wide spectrum of infections which includes skin and soft tissue infections, pneumonia, bacteraemia, surgical site infections (SSI), and catheter-related infections (De San et al 2007; Emily and Trish 2011)
All the MRSA isolates were subjected to the standard TCP assay and 22 tested isolates displayed biofilm positive phenotype in trypticase soya broth (TSB) medium, after incubation for 18 and 24 h, respectively
Optical density (OD) of stained adherent bacteria was determined with a micro-ELISA auto reader at wavelength of 570 nm (Table 1)

Summary

Introduction

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major nosocomial pathogens responsible for a wide spectrum of infections which includes skin and soft tissue infections, pneumonia, bacteraemia, surgical site infections (SSI), and catheter-related infections (De San et al 2007; Emily and Trish 2011). Biofilm formation is the main contributing factor to bacterial antibiotic resistance. Staphylococcus aureus becomes resistant by producing an extracellular polymeric substance (EPS) matrix that is composed of polysaccharides, nucleic acids, proteins and lipids (Davey and O’Toole 2000; Kokare et al 2009). Sessile bacteria embedded in this EPS matrix are able to withstand the host immune responses and thereby become less susceptible to antibiotics, which in turn fail to penetrate inside the biofilm. The most studied aspects of nanotechnology field are its ability to tussle bacterial infections through the production of nanoparticles (Luo et al 2007). The antimicrobial activities of metal oxide nanoparticles and their selective toxicity to biological systems suggest their potential

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