Antimicrobial activity of tropical and subtropical sponges

Abstract

Extracts of 19 of 24 species of sponge collected from Queensland. Australia, inhibited the growth of test microorganisms in in-vitro assays. A similar result has been obtained by earlier workers for extracts of species of sponge obtained from temperate waters. Some of the extracts, including extracts of Thorecta vasiforis, Arenochalina mirabilis and Acanthella kleutha, showed activity against bacterial strains that was comparable with that exhibited by penicillin G and streptomycin against these strains. Gram-positive bacteria were expecially sensitive to many extracts. Little activity was exhibited by any extract against four species of fungi tested. Some of the extracts were markedly toxic to one or more of the test organisms (a fish, a crustacean and a hydroid) used, but no clear pattern linking toxicity to these organisms with antimicrobial properties of the extracts emerged. In general, there bas a negative correlation between antimicrobial activity and surface-fouling, raising the possibility of using freedom from surface-fouling as an indicator of antimicrobial activity. Four of five encrusting species from the undersides of coral boulders showed antimicrobial activity. This study confirmed the value of using methanol-toluene extracts in field-based screening programmes, but emphasised the need to use additional extracting media such as methylene chloride in order to augment the number of antimicrobial compounds detected. There are indications that antimicrobial activity may be widespread in the order Dictyoceratida, in the order Verongida and in the order Axinellida, but sporadic in other orders of Porifera.