Abstract
The present study was carried out with an objective to investigate the antimicrobial potentials of leaves, flowers and stem extracts of Sphenoclea zeylanica. The aim of the study was to evaluate the antimicrobial activity and to determine the zone of inhibition of extracts against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using zone of inhibition method. The antibacterial and antifungal activities of extracts (40, 50, 60 and 70) of Sphenoclea zeylanica were tested against B. subtilis, P. vulgaris, S.aureus, E.coli, C. albicans, A. niger, S. typhi and B. cereus. Zone of inhibition of extracts were compared with that of control for antimicrobial activity. The results showed that the leaf extract showed notable inhibition of the microbial growth against the tested organisms. The microbial activity of the Sphenoclea zeylanic a might be due to the presence of various secondary metabolites. Hence, this plant can be used to identify the specific bioactive natural products which may serve as leads in the development of new antimicrobial agents.Int J Appl Sci Biotechnol, Vol 4(3): 325-329
Highlights
Medicinal plants are a rich source of antimicrobial agents and are used as a source of many potent drugs (Srivastava et al, 1996)
Active cultures of experiment were prepared by transferring a loop full of cells from the stock cultures to test tube of Muller-Hinton broth (MHB) for bacteria that were incubated without agitation for 24 hrs at 37oC and 25oC respectively
Eight organisms viz B. subtilis, P. vulgaris, S. aureus, E. coli, C. albicans, A. niger, S. typhi and B. cereus were taken as test organisms
Summary
Medicinal plants are a rich source of antimicrobial agents and are used as a source of many potent drugs (Srivastava et al, 1996). Stem, flower, fruit, twigs exudates and modified plant organs are used. While some of these raw drugs are collected by the local communities and folk healers in smaller quantities, but some raw drugs are traded in the market as the raw material for many herbal industries and collected in larger quantities (Uniyal et al, 2006). Herbal medicines are used by about 75-80% of whole population, and involves the use of plant extract and their active constituents (Akerele, 1993). Though conventional drugs are widely used, herbal medicines are utilized due the presence of secondary metabolites such as alkaloids, flavonoids, tannins, and terpenoids reported to have antibacterial activities (Cowan, 1999; Adenkunle and Adekunle, 2009; Lewis and Ausubel, 2006)
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