Abstract
We report here the characterisation of eight newly synthesized thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid and the evaluation of the in vitro antimicrobial activity of the new compounds against Gram-positive [Listeria monocytogenes, Staphylococcus aureus, Bacillus subtilis], Gram-negative [Psedomonas aeruginosa, Escherichia coli, Salmonella enteritidis], as well as Candida spp., using both reference and clinical multidrug resistant strains to establish the minimal inhibitory concentration (MIC) values. Our results showed that the tested compounds exhibited specific antimicrobial activities, both concerning the spectrum of antimicrobial activity and the corresponding MIC values, which ranged widely between 1024 and 32 μg/mL, depending on the nature and position of the substituents on the benzene ring. The most active compounds were N-[2-(4-chlorophenoxymethyl)-benzoyl]-N'-(2,6-dichlorophenyl)-thiourea (5g) and N-[2-(4-chlorophenoxymethyl)-benzoyl]-N'-(4-bromophenyl)-thiourea (5h), which showed a broad spectrum of antimicrobial activity against enterobacterial strains (E. coli and S. enteritidis), P. aeruginosa, S. aureus and Candida spp. All the tested compounds except 5f were highly active against S. aureus (MIC=32 μg/mL), suggesting their possible use in the treatment of MRSA infections. Four of compounds also exhibited antifungal activity (MIC =256-32 μg/mL) against C. albicans, but L. monocytogenes as well as B. subtilis were resistant to all tested compounds. Our studies thus demonstrated that among other biological activities, the thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid also exhibit selective and effective antimicrobial properties that could lead to the selection and use of these compounds as efficient antimicrobial agents, especially for the treatment of multidrug resistant infections.
Highlights
Thioureides are associated with a broad spectrum of biological activities including antibacterial, antifungal, tuberculostatic, anthelmintic, antitumor, anticonvulsant, diuretic, insecticidal and pesticidal properties
Our studies demonstrated that among other biological activities, the thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid exhibit selective and effective antimicrobial properties that could lead to the selection and use of these compounds as efficient antimicrobial agents, especially for the treatment of multidrug resistant infections
In light of the therapeutical importance of some N-acylated thiourea derivates, especially as antibacterials and antifungals, we have evaluated the in vitro activity of some new thioureides derived from 2-(4-chlorophenoxymethyl)-benzoic acid
Summary
Thioureides are associated with a broad spectrum of biological activities including antibacterial, antifungal, tuberculostatic, anthelmintic, antitumor, anticonvulsant, diuretic, insecticidal and pesticidal properties. In light of the therapeutical importance of some N-acylated thiourea derivates, especially as antibacterials and antifungals, we have evaluated the in vitro activity of some new thioureides derived from 2-(4-chlorophenoxymethyl)-benzoic acid. We have focused on a qualitative screening assay of the antimicrobial properties (adapted disk diffusion method), as well as a quantitative assay of the antimicrobial activity (binary microdilution method), to establish the minimal inhibitory concentration (MIC) values of these compounds against microbial Gram-positive, Gram-negative and fungal strains. We have extended our research to the preparation and evaluation of the antimicrobial activity of some new thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid. The reaction conditions, yields and some of the physical properties were established. NMR and IR spectral parameters and the elemental analysis have confirmed the structure of the synthesized compounds
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