Abstract
Acinetobacter baumannii is one of the most challenging pathogens, on account of its predisposition to develop resistance leading to severe, difficult-to-treat infections. As these bacteria are more usually isolated from nosocomial infections, the new therapeutic options are demanded. Antimicrobial peptides (AMPs) are compounds likely to find application in the treatment of A. baumannii. These compounds exhibit a wide spectrum of antimicrobial activity and were found to be effective against biofilm. In this study, eight AMPs, namely aurein 1.2, CAMEL, citropin 1.1., LL-37, omiganan, r-omiganan, pexiganan, and temporin A, were tested for their antimicrobial activity. A reference strain of A. baumannii ATCC 19606 was used. Antimicrobial assays included determination of the minimum inhibitory concentration and the minimum biofilm eradication concentration. Considering the fact that the majority of A. baumannii infections are associated with mechanical ventilation and the use of indwelling devices, the activity against biofilm was assessed on both a polystyrene surface and tracheal tube fragments. In addition, cytotoxicity (HaCaT) was determined and in vitro selectivity index was calculated.
Highlights
The Acinetobacter bacteria are currently one of the major causes of nosocomial infections while Acinetobacter baumannii is considered to be the major pathogen, owing to its predisposition to develop resistance [1]
The aim of this study was to evaluate the activity of eight antimicrobial peptides (AMPs), namely aurein 1.2, CAMEL, citropin 1.1., LL-37, omiganan, r-omiganan, pexiganan, and temporin A, against reference a strain of A. baumannii ATCC 19606
Eight different AMPs and six conventional antibiotics were tested against the reference strain of A. baumannii ATCC 19606
Summary
The Acinetobacter bacteria are currently one of the major causes of nosocomial infections while Acinetobacter baumannii is considered to be the major pathogen, owing to its predisposition to develop resistance [1]. These aerobic, Gram-negative bacteria have been routinely isolated form nosocomial infections, notably from patients of intensive care units [2] These infections are frequently severe and difficult to treat, on account of their correlation with inter-regional spread and local occurrence of A. baumannii resistant to carbapenems [3]. Antimicrobial peptides are an interesting class of compounds that can be an alternative to conventional antibiotics These endogenic molecules are widely distributed in nature, mainly as a part of innate immunity of organisms [12]. They target a broad spectrum of pathogens (bacteria, fungi, protozoa, and viruses) and are associated with triggering and coordinating multiple components of innate and immune adaptive systems [13,14,15,16] Owing to these properties, antimicrobial peptides (AMPs) have attracted much attention of researchers from many scientific groups worldwide.
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