Antimicrobial activity of phytoextracts on opportunistic oral bacteria, yeast and bacteria from probiotics

H M Semenova,H V Yavorska,M V Kryvtsova,M Y Spivak,N M Vorobets,O Y Rivis

doi:10.15421/021855

Abstract

Developed experimental assays enable us to compare the antimicrobial activity of herbal medicinal drugs on Lactobacillus and Bacillus strains probiotics, which have been claimed to possess the ability of suppressing the growth of various oral pathogens. In the treatment of periodontal disease it is advisable to use a comprehensive approach which would include the application of herbal remedies and probiotics. The combination of such effects may be a new approach in dentistry due to their complementary antimicrobial activity. In this study, we researched antimicrobial effects of herbal medicinal drugs (tinctures of some medical plants, solutions Rotocanum and Chlorophyllipt) against collection strains and clinical strains isolated from the oral cavity of patients with periodontitis, and probiotic strains Bacillus subtilis UKM В-5007 and Bacillus licheniformis UKM В-5514 that are part of the active base of probiotic Biosporin (Ukraine), Bacillus clausii from the probiotic Normaflore (Hungary), as well as the strains Lactobacillus spp. – from probiotic Lactobacterinum (Biopharma, Ukraine). For investigation, the standard agar dilution method was used in modification with glass cylinders. The results of the research showed that among the studied herbal medicinal remedies, tinctures of Eucalypthus viminalis, Mentha piperita and Chlorophyllipt had the strongest antimicrobial activity. Probiotic strains are also sensitive to herbal tinctures (except the tinctura of wormwood), which indicate the possibility of only consecutive usage (with an interval of time) of herbal remedies and probiotics in combination therapy in the treatment of periodontal diseases.

Highlights

Oral health influences the general quality of life, and poor oral health is linked to chronic conditions and systemic diseases
To determine the antimicrobial activity of the herbal medicinal remedies, as test cultures we used bacteria from the American Type Culture Collection, USA: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, and yeast Candida albicans ATCC 885-653; clinical strains of bacteria: Staphylococcus aureus, Streptococcus salivarius, Enterobacter sp., Neisseria sp.; yeast Candida albicans, isolated from the oral cavity of periodontitis patients; and bacteria Bacillus subtilis UKM В-5007 and Bacillus licheniformis UKM В-5514, isolated from probiotic Biosporin (Biopharma, Ukraine), and Bacillus clausii from the probiotic Normaflore (Manufacturer: Uniter Laboratory, France; holder of Sanofi-aventis S.p.A., Hungary trade license), as well as the strains Lactobacillus spp. – from probiotic Lactobacterinum (Biopharma, Ukraine)
Research has shown that Tinctura Eucalipti has high antimicrobial activity against Grampositive, Gram-negative microorganisms, and probiotic strains

Summary

Introduction

Oral health influences the general quality of life, and poor oral health is linked to chronic conditions and systemic diseases. Treatment of patients with periodontal diseases should include both local and general therapy It must be based on the understanding of the mechanisms of action of the pharmaceutical or prophylactic agents, and be used to be effective and at the same time safe for the patient. The drugs of plant origin correspond to such criteria, because active compounds in their composition can act bacteriostatically and bactericidally (Rios et al, 2005; Kačániováa et al, 2014; Hleba et al, 2016). Another group of medicines – probiotics have relatively recently started to be used for periodontal treatment. Probiotics are commonly bacteria from genera Lactobacillus (including strains L. salivarius and L. plantarum) and Bifidobacterium (including strains B. bifidum, B. longum) or others, which could be beneficial to the digestive system, but to oral health too (Mishra et al, 2014; Alok et al, 2017)

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