Antimicrobial activity of leaf and root methanolic extracts from Vinca pusilla Murr.

Gireesh Ankad,S Hoti,Subarna Roy,Harsha Pednekar,Harsha Hegde,Sandeep Pai

doi:10.14719/pst.2016.3.1.163

Abstract

Vinca pusilla Murr. is a traditional medicinal plant used to treat several diseases. To substantiate the traditional medicinal utility of the plant, the present study aims at screening the antimicrobial activity of methanolic extracts of leaves and roots against five Gram positive, five Gram negative bacterial and four fungal strains. The minimum inhibitory concentration (MIC) were determined by two fold dilution assay. The results indicated that, leaf and root extracts were more effective on Bacillus subtilis and Staphylococcus aureus strains (MIC < 1 mg/mL). The tested organisms were sensitive to root extract compared to leaf extract. Fungal strains were resistant than the bacterial strains to both the extracts. Thus the present study illustrates the antimicrobial potential of the plant.

Highlights

Antimicrobial activity tested were obtained as minimum inhibitory concentration (MIC)
Both extracts inhibited the growth of organisms with varying degree of MIC confirming antimicrobial property
Among Gram–ve bacteria, P. aeruginosa was more effectively inhibited by root extract with MIC value 1.34 ± 0.75 mg/mL, while other bacteria were inhibited by extracts with higher MIC values (> 2 mg/mL)

Summary

Introduction

Keywords Vinca pusilla; traditional medicine; methanolic extract; antimicrobial; Bacillus subtilis; Staphylococcus aureus Antimicrobial activity of leaf and root methanolic extracts from Vinca pusilla Murr. Initiatives to screen several medicinal plants for their antimicrobial activity has been emphasized (Cushnie and Lamb, 2005). The exercise of screening antimicrobial activities of plants has already provided modern medicine with an abundance of drugs and treatments against the infectious diseases (Ankad et al, 2014).

Results

Conclusion