ANTIMICROBIAL ACTIVITY OF LACTOBACILLI AGAINST STAPHYLOCOCCI ISOLATED FROM BIOFILMS FROM PATIENTS WITH ACNE VULGARIS

Abstract

Aim. Determine the effectiveness of the antimicrobial activity of Lactobacillus isolates against biofilm producing staphylococci in vitro. Methods. There were used 15 strains of biofilm producing staphylococci isolated from purulent pustules of patients with acne vulgaris and 7 strains of lactobacilli. L. fermentum (n = 3) were obtained from oropharyngeal mucus, L. plantаrum (n = 3) from the feces of practically healthy individuals, probiotic strain L. plantarum 8P-A3 from the drug "Lactobacterin" (Biopharma, Kyiv).The antimicrobial activity of bacterial cultures of lactobacilli and filtered supernatant liquid of lactobacilli were studied using biofilm producing S. aureus strains by agar diffusion method after 12, 24 and 48 hours. Results. The antagonistic activity of lactobacilli was determined by 15 strains of S. aureus in which icaA and icaD genes were detected. For all strains of L. plantаrum (12 h), high antagonistic activity was established and an average antagonistic activity for L. fermentum was established. In 24-h, in the studied lactobacilli cultures, there was observed a tendency of decreasing their activity. After 48 hours, a significant decrease in staphylococci growth inhibition zones was observed in comparison with the results of 12-hour cultures – on average 7.9 ± 0.2 mm versus 12.5 ± 0.7 mm, respectively. Filtration of the supernatant liquid of lactobacilli (12 h) led to a sharp decrease in their activity by 2.8 times compared with the rates of native cultures of lactobacilli. Conclusions. The antibacterial activity of native bacterial cultures of lactobacilli against biofilm producing S. aureus reaches a maximum level after 12 hours. The minimal inhibitory effects of the filtered supernatant liquid of lactobacilli against biofilm producing strains of S. aureus were established. Isolates of L. plantarum showed higher antagonistic activity against staphylococci than L. fermentum, and may be proposed as an option for the development of combination therapy of acne.