Antimicrobial activity of lactiplantibacillus plantarum against shiga toxin-producing escherichia coli.

Abstract

The aim of the present work was to characterize Lactiplantibacillus sp. LP5 strain, isolated from pork production, and identify bacteriocin-like inhibitory substances produced by this strain. In this study, LP5 was identified by specie-specific PCR and 16S rDNA sequencing. Additionally, bacterial growth kinetics, antimicrobial activity, detection of the genes related to plantaricins production and genetic expression of plantaricins, were determined. Lactiplantibacillus sp. LP5 was identified as Lactiplantibacillus plantarum. The well-diffusion test using cell-free supernatants (CFS), neutralized CFS, CFS treated with catalase and CFS treated with proteinase K showed that inhibitory effects on a Shiga toxin-producing E. coli (STEC) strain were produced by bacteriocins. The PCR technique allowed the detection of genes encoding E/F plantaricins, as well as J/K and whole genome sequencing and bacteriocin mining analysis allowed us to confirm the presence of these plantaricins. We can conclude that the inhibitory effect of L. plantarum LP5 isolated from pigs against STEC EDL933 strain could be associated with the bacteriocins production and represents a potential use as a probiotic strain.