Antimicrobial activity of Ginkgo biloba leaf extract on Listeria monocytogenes

Abstract

ABSTRACT: The antimicrobial activities of Ginkgo biloba leaf extract (GBE) and the combined effects of GBE and sodium EDTA (sodium Ethylenediaminetetraacetic acid) against Listeria monocytogenes were determined at 4 °C, 25 °C, and 37 °C. Listeria monocytogenes grown at 37 °C for 24 h was inoculated (6 to 7 log CFU/mL) into BHI broth containing either GBE or GBE and EDTA (1.6 mg/mL) with various GBE concentrations of 0.1, 0.25, 0.5, 1, 2.5, 5.0, 7.5, 10.0, 15.0, or 20.0% vol/vol and stored at 4 °C, 25 °C, and 37 °C. The inhibitory effect of the GBE was more pronounced at low temperature of 4 °C. GBE was effective in inhibiting microbial growth. Addition of EDTA enhanced antimicrobial activity of GBE.