Abstract
Antimicrobial Activity of Extracellular Proteins Secreted by Bacillus Subtilis ATCC21332 after being Induced with Cymbopogon Flexuosus Essential Oil and Cultured in Different Growth Conditions
Highlights
Multidrug-resistance against pathogenic bacteria has been increased alarmingly during the last few decades due to the over usage
The extracellular proteins produced by bacterial cells in the presence of C. flexuosus essential oil and grow in normal pH without media modification was study for antimicrobial activity
The extracellular proteins secreted by B. subtilis ATCC21332 prior to treatment with 0.01 minimal inhibitory concentration (MIC) of C. flexuosus essential oil and grown in media with 1% of sucrose showed antimicrobial activity towards two Grampositive bacterial strains which are S. epidermidis and B. cereus with each size of inhibition zone 8.0 ± 0.8 mm and 6.0 ± 0.0 mm respectively
Summary
Multidrug-resistance against pathogenic bacteria has been increased alarmingly during the last few decades due to the over usage. Antimicrobial proteins or peptides (AMPs) are gaining greater attention as alternative therapeutics against antibiotic resistant pathogens and spoilage bacteria. Antimicrobial agents are bioactive compounds that can serve as weapon due to their inhibitory activity toward other microorganisms In ecological environments, these compounds may be at lower concentrations and likely play additional roles as signalling molecules [10]. These compounds may be at lower concentrations and likely play additional roles as signalling molecules [10] It appears that many antimicrobial agents, when used at low concentrations, have the ability to activate gene transcription, which is distinct from their inhibitory effect [11]. Attempt has been made to determine the antimicrobial activity of extracellular proteins produced by Bacillus sp. during mild stress condition in the presence of C. flexuosus essential oil and changes of growth condition
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