Abstract
AimsThe aim of this study was to determine the relative antimicrobial effectiveness of these endodontic medicaments and various vehicles using an agar well diffusion assay.Materials and methodsDouble Antibiotic Paste(DAP), modified DAP, 2% Chlorhexidine gluconate and their combination with four vehicles namely Polyethylene glycol 400 (PEG), Propylene glycol (PG), combinations of PG with PEG and lastly Glycerine were tested using agar well diffusion assay. The minimum bactericidal concentration was noted against four standard strains of organisms ie Streptococcus mutans ATCC( American Type Culture Collection) 25175, Staphylococcus aureus ATCC 12598, Enterococcus faecalis ATCC 35550 and Eschericia coli ATCC 25922. Successful endodontic therapy depends upon thorough disinfection of root canals. In some refractory cases, routine endodontic therapy is not sufficient, so intracanal medicaments are used for proper disinfection of canals. Issues of resistance, limited spectrum of activity and lack of antifungal properties, the hunt for the ideal intracanal medicament continues. In this regard, the vehicles used to form the pastes play a supportive role by forming the appropriate consistency for placement and may dramatically influence their chemical characteristics like their solubility and diffusion. Thus, inorder to use safer and equally effective intracanal medicaments, Chlorhexidine gluconate is being unveiled in this study.ResultsThe difference between the four vehicles when combined with the same endodontic medicament studied above is nonsignificant (NS) except against Porphyromonas gingivalis. Propylene glycol is significantly effective than Glycerine when used with DAP ie C+M medicament combination. (p = 0.029)Conclusion2% chlorhexidine gluconate and modified DAP can definitely replace DAP and triple antibiotic paste as end-odontic medicaments with chlorhexidine having an added advantage of bactericidal action, substantivity, biocompatibility, low toxicity, and lesser chances of developing resistance.How to cite this articleNalawade TM, Bhat KG, Sogi S. Antimicrobial Activity of Endodontic Medicaments and Vehicles using Agar Well Diffusion Method on Facultative and Obligate Anaerobes. Int J Clin Pediatr Dent 2016;9(4):335-341.
Highlights
Dental caries is the most common chronic disease and one of the most expensive diseases to treat
The difference between the four vehicles when combined with the same endodontic medicament studied above is nonsignificant (NS) except against Porphyromonas gingivalis
The least Minimum inhibitory concentration (MIC) value of all medicaments was that of chlorhexidine and all organisms were resistant to metronidazole (Table 1)
Summary
Dental caries is the most common chronic disease and one of the most expensive diseases to treat. As per the National Oral Health Survey and Fluoride Mapping (2002–2003), there is a very high proportion of untreated caries.[1] Successful endodontic therapy of the teeth affected with dental caries consists of thorough disinfections of the root canals which cannot be attained by standard treatment alone. The use of endodontic medicaments for sterilization of root canals especially resistant microbes like Enterococcus faecalis has become a necessity. Minocycline in TAP has been associated with discoloration and chances of affecting the developing permanent successor if used in deciduous teeth like other tetracyclines cannot be denied.[2] Appropriate use of the existing antibiotics and antimicrobial agents and their combinations, which help in decreasing the incidence of resistance development, should be tested and incorporated in the treatment of infectious diseases
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