Antimicrobial activity of different dental cements on aggregatibacter actinomycetemcomitans

Abstract

Objectives: The objective of this study was to evaluate, in vitro, the possible antimicrobial activity against the A actinomycetemcomytans of three dental cements: glass-ionomer cement, zinc phosphate cement and resin cement. Material and Methods: Strains of A actinomycetemcomitans ATCC 29522 were used. The microorganism was grown in BHI Agar and transferred to tubes containing sterile saline solution. The suspension was calibrated to a similar turbidity to the 0.5 tube from McFarland scale. A base layer consisting of 20 ml of BHI agar was placed in sterile Petri 90 x 15 mm plates. After solidification, was added 0,1 uL of microbial suspension, and three wells with 6 mm in diameter and 1mm deep were made, the wells were filled with cements with zinc phosphate cement, glass-ionomer cement and resin cement indicated for permanent cementation for fixed prostheses immediately after handling and positive (chlorhexidine) and negative (saline) controls. Results: After 48 hours the presence or absence of inhibition halo of microbial growth was analyzed around the specimens. Conclusion: Zinc phosphate cement and glass ionomer cement tested showed antibacterial activity against A actinomycetemcomitans unlike resin cement.